Evidence map›Paper›PMID 42310333›Full record

ArticleScientific reports2026

Dietary cauliflower (Brassica oleracea var. botrytis) mitigates benzo[a]pyrene-induced oxidative stress, immune dysfunction, and tissue damage in Nile tilapia.

Zeinab El-Bouhy, Afaf N Abdel Rahman, Fatma A S Mohamed, Mohamed W A Elashhab, Tarek Khamis, Rasha M Reda

Erratum issuedAbstract read
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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Zeinab El-BouhyDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt.
Afaf N Abdel RahmanDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt. afne56@gmail.com.
Fatma A S MohamedDepartment of Aquatic Health and Diseases, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
Mohamed W A ElashhabDepartment of Aquatic Health and Diseases, National Institute of Oceanography and Fisheries, NIOF, Cairo, Egypt.
Tarek KhamisDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt.
Rasha M RedaDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Benzo[a]pyrene (BaP) is a ubiquitous polycyclic aromatic hydrocarbon that has been reported to induce immunotoxicity and oxidative stress in fish. In the current study, the potential protective efficacy of dietary cauliflower powder (CAF; Brassica oleracea var. botrytis) is investigated in Nile tilapia (Oreochromis niloticus) subjected to water-borne BaP exposure. Fish (initial body weight 35.45 ± 5.33 g; n = 280) were randomly assigned to seven groups (n = 40/group): control, acetone (12.25 µL/L), CAF0.5 (0.5%), CAF1 (1%), BaP (12.25 µg/L), CAF0.5 + BaP, and CAF1 + BaP, and maintained on their respective regimens for 30 days. Exposure to BaP induced 30% cumulative mortality, accompanied by distinct clinical-behavioral abnormalities, hepato-renal dysfunction, and depleted total protein fractions. Concurrently, stress biomarkers (glucose and cortisol) and lipid peroxidation (malondialdehyde) were markedly elevated. BaP exposure also suppressed antioxidant-immune responses, caused severe hematological alterations, disrupted splenic gene expression, and provoked pronounced histopathological lesions across multiple tissues (liver, brain, gills, and muscle). This damage correlated with high BaP bioaccumulation in muscle tissue and compromised resistance against Aeromonas hydrophila infection. Remarkably, dietary CAF intervention, particularly at the 1% inclusion level, significantly counteracted these toxic effects (P < 0.05). CAF supplementation reduced mortality, restored biochemical and immune profiles, lowered muscle BaP residues, and partially preserved normal tissue architecture. Furthermore, CAF-fed fish exhibited significantly enhanced post-challenge survival rates against A. hydrophila. Overall, these findings demonstrate that dietary CAF supplementation serves as an effective nutritional strategy to mitigate BaP-induced toxicity and support fish health in contaminated aquaculture environments.

Indexed as

Benzo(a)pyreneBrassicaCichlidsOxidative StressAnimal FeedAnimalsDietFish DiseasesLipid PeroxidationBenzo(a)pyreneBenzo[a]pyreneCauliflowerFeed additivesGenotoxicityNile tilapiaPhysiological status

Identifiers

PMID42310333
PMCPMC13276132

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.