In one paragraphArticle in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
9 authors.
Carlos Cal Y Mayor Luna *Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA.ORCID 0000-0002-2109-0530 Martín Alcorlo *Department of Crystallography and Structural Biology, Instituto de Química-Física "Blas Cabrera", Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Giselle N JacobsonBiophysics Instrumentation Core Facility, University of Notre Dame, Notre Dame, IN, USA.
Melisa LázaroCenter for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Derio, Spain.ORCID 0000-0002-9603-9115 Mikel ValleCenter for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Bizkaia Technology Park, Derio, Spain.ORCID 0000-0001-8268-6912 Mayland ChangDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA. mchang@nd.edu.ORCID 0000-0002-4333-3775 Juan A HermosoDepartment of Crystallography and Structural Biology, Instituto de Química-Física "Blas Cabrera", Consejo Superior de Investigaciones Científicas, Madrid, Spain. xjuan@iqf.csic.es.ORCID 0000-0002-1862-8950 Shahriar MobasheryDepartment of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA. mobashery@nd.edu.ORCID 0000-0002-7695-7883 Funding
Chemistry-Biochemistry-Biology Interface (CBBI) Program at Notre DameT32GM145773 · NIGMS · UNIVERSITY OF NOTRE DAME · PI Christian Corey Melander · 2022 to 2026
$1.5MSpore germination pathway in Clostridioides difficile and its pharmacological interventionR01AI178776 · NIAID · UNIVERSITY OF NOTRE DAME · PI CHANG, MAYLAND F · 2024 to 2024
$771kClostridiodes difficile spore germination and therapeutic interventionR01AI198244 · NIAID · UNIVERSITY OF NOTRE DAME · PI Mayland F Chang · 2026 to 2026
$769kDivision of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) AI178776Division of Intramural Research, National Institute of Allergy and Infectious Diseases (Division of Intramural Research of the NIAID) AI198244NIAID NIH HHS R01 AI178776NIAID NIH HHS R01 AI198244NIGMS NIH HHS T32 GM145773Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) CRSII5_198737/1Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) MCIN/AEI/10.13039/501100011033Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) PID2021-124074NB-I00Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) PID2023-153118OB-I00U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) T32GM145773
6 · The paper itselfAbstract
The germinosome is the machinery of Clostridioides difficile that sets in motion the process of spore germination to vegetative bacteria. Three highly-regulated proteins-CspA, CspB and CspC-serve as key instigators of germination. We report that CspA and CspB exist independently as homodimers in solution. In the presence of CspC, a picomolar complex of CspA:CspC forms. Furthermore, we document that CspA binds to the germinant, taurocholate, and that the complex CspA:CspC:taurocholate serves as the receptor for glycine, the co-germinant. We report high-resolution X-ray and cryo-EM structures for the three proteins, and for the CspA:CspC:taurocholate complex. These structures show how the CspA:CspC heterodimer recognizes taurocholate and reveal that specific structural features in the three Csp proteins avoid the recognition of the germinant by homodimers. Remarkably, the homodimer of CspB organizes itself in a supramolecular fibril assembly comprised of a three-stranded right-handed superhelix. These proteins are targets for interference with the transition from spore to the vegetative form of C. difficile.
Indexed as
Bacterial ProteinsClostridioides difficileSpores, BacterialCryoelectron MicroscopyCrystallography, X-RayModels, MolecularProtein BindingProtein MultimerizationTaurocholic AcidBacterial Proteinscold-shock protein CspB, BacteriaTaurocholic Acid
Identifiers
PMID42310002
PMCPMC13434707
What OpenQuestion holds
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LicenceCC BY
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