ArticleJournal, genetic engineering & biotechnology2026
Development and validation of a quantitative pcr array assay for the detection of viral sequences in breast cancer.
Article in Journal, genetic engineering & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo develop and validate a quantitative PCR Multiplex Array assay for the detection of viral sequences in breast cancer (BC) tissue, and to assess its potential utility as a molecular research and diagnostic tool.
methodsA total of 172 formalin-fixed paraffin-embedded (FFPE) biopsy samples from patients with a histological diagnosis of BC, and 10 non-neoplastic breast tissue samples were collected at the High Specialty Regional Hospital "Ciudad Salud" (HRAECS). The study was approved by the Institutional Research and Ethics Committees. Samples were analyzed using a qPCR Multiplex Array assay. gBlocks Gene Fragments at 10 ng/µL were used as positive controls for each target virus.
resultsAssay validation demonstrated high specificity, linearity (R
conclusionA qPCR Multiplex Array assay targeting 21 viral sequences was developed and validated, providing a reliable tool for the detection of cancer-associated viral sequences in BC tissue. Detection of viral sequences confirms their presence in tumor tissue but does not establish causality with carcinogenesis. Future studies integrating transcriptomic, functional, and clinical analyses are needed to clarify the role of these viruses in BC, including associations with clinicopathological features and potential therapeutic implications.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.