ArticleJournal, genetic engineering & biotechnology2026
Phytochemical profile, antioxidant, antimicrobial activity, antibiofilm, cytotoxicity, and HR-LCMS of Tribulus terrestris.
Article in Journal, genetic engineering & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundTribulus Terrestris (TT) is a Phytotherapeutic species employed in ethanopharmacological practices, stimulating spermatogenic and androgenic pathways, enhancing stamina, and promoting prophylaxis of nephrolithiasis. In this study, the focus will be on the phytochemical composition, of (TT), that contributes to antioxidant activity, antimicrobial, anti-biofilm properties.
methodsThe ethanolic extract of TT (ETT) was obtained through the Soxhlet extraction process, which was subsequently followed by rotary evaporation and lyophilization. A preliminary screening for phytochemicals was conducted, and the antioxidant activity was assessed using DPPH FRAP, and ABTS assays. Antimicrobial and antibiofilm assays were conducted. The cytotoxic effects of the ETT were evaluated utilizing L929 and HEK-293 cell lines. Scanning electron microscopy (SEM) was used to observe the morphological changes in bacteria. HR-LCMS analysis was conducted to identify its bioactive compounds.
resultsPhytochemical screening verified the existence of phenolics, alkaloids, saponins, glycosides, flavonoids, carbohydrates, proteins, amino acids, tannins, and steroids. The phenolic concentration was measured at 7.96 µg/mL, while the flavonoid concentration was found to be 23.22 µg/ml. The ethanolic extract exhibited antioxidant activity with IC
conclusionThe multifaceted bioactivity of (TT) ethanolic extract, such as antioxidant activity, antimicrobial, and anti-biofilm inhibition, stems from its diverse phytochemical metabolites. This underscores their relevance in traditional ayurvedic practices and validates their.
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