ArticleSTAR protocols2026
Protocol for total internal reflection fluorescence microscopy image preprocessing and radial analysis of LFA-1 in T cell immune synapses.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Here, we present a protocol for total internal reflection fluorescence (TIRF) microscopy image preprocessing and radial analysis of lymphocyte function-associated antigen-1 (LFA-1) in T cell immune synapses. We describe steps for combining customizable Fiji/ImageJ macros for image background subtraction and segmentation with flexible R-based scripts for peripheral supramolecular activation cluster (pSMAC) center identification, center-based cropping, and radial analysis of synaptic LFA-1 in T cells. This workflow supports established analyses, including radial averaging/spinning and fluorescence intensity measurements across the synapse.
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