Evidence map›Paper›PMID 42307078›Full record

ArticleBiotechnology journal2026

Severe Restriction of Glucose Import Enhances Recombinant Protein Production in Escherichia coli.

Juan Carlos Fragoso-Jiménez, Juan Carlos Sigala, Frank Delvigne, Alfredo Martínez, Alvaro R Lara, Guillermo Gosset

Abstract read
In one paragraph

Article in Biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Juan Carlos Fragoso-JiménezInstituto de Biotecnología, Departamento de Ingeniería Celular y Biocatálisis, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Juan Carlos SigalaDepartamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, México DF, México.
Frank DelvigneTerra Research and Teaching Centre, Microbial Processes and Interactions, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.ORCID https://orcid.org/0000-0002-1679-1914
Alfredo MartínezInstituto de Biotecnología, Departamento de Ingeniería Celular y Biocatálisis, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.
Alvaro R LaraDepartment of Biological and Chemical Engineering, Aarhus University, Aarhus, Denmark.ORCID https://orcid.org/0000-0003-3535-7619
Guillermo GossetInstituto de Biotecnología, Departamento de Ingeniería Celular y Biocatálisis, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, México.ORCID https://orcid.org/0000-0003-3127-0911

Funding

CONAHCyT A1-S-8646CONAHCyT SECIHTI (Mexico)Consejo nacional de humanidades, ciencias y tecnologia
6 · The paper itself

Abstract

Glucose uptake through the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) enables rapid growth of Escherichia coli but promotes overflow metabolism that limits recombinant protein production. To define how glucose transport capacity shapes cellular physiology and bioprocess performance, we compared wild-type W3110 with two PTS-deficient derivatives, VH33 (ΔptsHIcrr, constitutive galP expression) and WHIC (ΔptsHIcrr ΔmglABC), in controlled stirred-tank bioreactors containing 20 g L

Indexed as

Escherichia coliGlucoseRecombinant ProteinsAcetatesBioreactorsEscherichia coli ProteinsMetabolic EngineeringPhosphoenolpyruvate Sugar Phosphotransferase SystemAcetatesEscherichia coli ProteinsGlucosePhosphoenolpyruvate Sugar Phosphotransferase SystemRecombinant Proteinsacetate overflowglucose importmetabolic engineeringphosphoenolpyruvate:carbohydrate phosphotransferase system (PTS)recombinant protein productiontranscriptomics

Identifiers

PMID42307078
PMCPMC13274328

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.