In vitro antifungal resistance and molecular analysis of the SQLE gene in dermatophytosis: a laboratory-based study.

Jegan Charlies, Latha Ragunathan, Kavitha Kanniyan, Jeyakumari Duraipandian

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Article in Iranian journal of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Jegan CharliesDepartment of Microbiology, Vinayaka Mission's Medical College and Hospital, Vinayaka Mission's Research Foundation (Deemed-to-be-University), Karaikal, Puducherry, India.

Latha RagunathanDepartment of Microbiology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission's Research Foundation, (Deemed-to-be-University), Kirumampakkam, Puducherry, India.

Kavitha KanniyanDepartment of Microbiology, Aarupadai Veedu Medical College and Hospital, Vinayaka Mission's Research Foundation, (Deemed-to-be-University), Kirumampakkam, Puducherry, India.

Jeyakumari DuraipandianDepartment of Microbiology, JIPMER, Karaikal, Puducherry, India.

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6 · The paper itself

Abstract

Background and Objectives: Dermatophytosis is a common superficial fungal infection that affects the keratinised tissues. This study examines the antifungal resistance mechanisms and molecular detection of squalene epoxidase gene alterations in clinical isolates from patients. Materials and Methods: A cross-sectional study conducted from January 2019 to December 2020 included 110 clinically suspected dermatophytosis specimens. Microscopy, culture, CLSI M38-A2 broth microdilution testing, and PCR-based ITS and SQLE (520 bp) amplification were performed. MIC50, MIC90, geometric mean, and ranges were analysed using SPSS version 19 with P < 0.05. Results: A total of 110 suspected cases were evaluated, with 78 (70.9%) males. KOH positivity reached 100%, while culture positivity was 47.3% (52/110). Conclusion: Terbinafine and caspofungin showed consistent in vitro activity, while fluconazole showed limited activity. Routine susceptibility testing guides drug selection, improves treatment outcomes, and supports rational management of resistant dermatophytosis cases.

Indexed as

Antifungal resistanceGene expressionMinimum inhibitory concentrationPolymerase chain reactionSqualene epoxidase

Identifiers

PMID42306509

DOI10.18502/ijm.v18i3.21667

PMCPMC13265854

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