Evidence map›Paper›PMID 42305586›Full record

ArticleiScience2026

3D EM uncovers mitochondrial network remodeling in residual triple negative breast cancer after conventional chemotherapy treatments.

Mariah J Berner, Heather K Beasley, Benjamin Rodriguez, Audra Lane, Steven W Wall, Andrea G Marshall, Zer Vue, Larry Vang, Mokryun L Baek, Mason Killion and 15 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

25 authors.

Mariah J BernerLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Benjamin RodriguezDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Audra LaneLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Steven W WallLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Mokryun L BaekLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Mason KillionDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Faben ZelekeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Bryanna ShaoDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Dominique ParkerDepartment of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, USA.
Autumn PetersonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Julie Sterling RhoadesDepartment of Veterans Affairs, Tennessee Valley Healthcare System, Nashville, TN, USA.
Lacey E DobroleckiLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Annet KiraboDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Sepiso K MasengaDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Prasanna KattiDepartment of Biology, Indian Institute of Science Education and Research (IISER), Tirupati, AP, India.
Prasanna VenkhateshDepartment of Biology, Indian Institute of Science Education and Research (IISER), Tirupati, AP, India.
Chandravanu DashThe Center for AIDS Health Disparities Research, Meharry Medical College, Nashville, TN, USA.
Michael T LewisLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.
Antentor HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA.
Gloria V EcheverriaLester and Sue Smith Breast Cancer, Baylor College of Medicine, Houston, TX, USA.

Funding

The RCMI Program in Health Disparities Research at Meharry Medical College - SupplementU54MD007586 · NIMHD · MEHARRY MEDICAL COLLEGE · PI Samuel Evans Adunyah · 2017 to 2026
$48.2M
Tennessee CFAR: Implementation of Culturally Responsive Trauma-Informed Care with Youth with HIV in Memphis, TNP30AI110527 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI John Koethe · 2015 to 2026
$27.5M
Role of GRIN2 in ART and SUD associated neurological deficits.R01DA057204 · NIDA · MEHARRY MEDICAL COLLEGE · PI Chandravanu Dash · 2022 to 2026
$3.7M
Spatiotemporal Staging of the HIV-1 Preintegration complexR01AI170228 · NIAID · MEHARRY MEDICAL COLLEGE · PI Chandravanu Dash · 2022 to 2026
$2.8M
Role of the Viral Capsid in HIV-1 IntegrationR01AI136740 · NIAID · MEHARRY MEDICAL COLLEGE · PI Chandravanu Dash · 2019 to 2026
$2.2M
Enhancing Virology Training of Underrepresented Minority Students through Summer ResearchR25AI164610 · NIAID · MEHARRY MEDICAL COLLEGE · PI DASH, CHANDRAVANU · 2021 to 2025
$1.8M
NIAID NIH HHS P30 AI110527NIAID NIH HHS R01 AI136740NIAID NIH HHS R01 AI170228NIAID NIH HHS R25 AI164610NIDA NIH HHS R01 DA057204NIMHD NIH HHS U54 MD007586
6 · The paper itself

Abstract

Mitochondria are hubs of metabolism and signaling. We previously demonstrated the importance of mitochondrial structure and function in chemotherapy-refractory triple-negative breast cancer (TNBC). Herein, we present the first 3D analysis of mitochondrial networks in human tumor tissues. Using serial block face scanning electron microscopy, we reconstructed 3,750 mitochondria and 800 lipid droplets (LDs) in naive and residual tumors persisting after conventional chemotherapies in two orthotopic patient-derived xenografts (PDX). Chemotherapies administered as monotherapy or in combination produced residual tumors that harbored mitochondria with significantly increased areas, volumes, and perimeters. We observed substantial reduction of mitochondrial intratumor heterogeneity following all treatments. Further, mitochondrial complexity was significantly elevated after single-agent treatments in one model, but was reduced in the other PDX model. Mitochondria-LD significantly increased contacts in residual tumors, congruent with our previous studies providing evidence for rewiring of lipid metabolism in residual TNBC. These results highlight the potential for structure-based monitoring of chemotherapy-induced metabolic rewiring in TNBC.

Indexed as

BiochemistryCell biologyMedical imagingOncologyPathologyPharmacology

Identifiers

PMID42305586
PMCPMC13266026

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.