Evidence map›Paper›PMID 42305134›Full record

ArticleBio-protocol2026

ROOT-ExM: Super-Resolution Imaging of Proteins in

Yutaro Shimizu, Guillaume Maucort, Mónica Fernández-Monreal, Florentin Dumas, Emmanuelle M Bayer, Yohann Boutté, Magali S Grison

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In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yutaro ShimizuLaboratoire de Biogenèse Membranaire, Université de Bordeaux, UMR5200 CNRS, Villenave d'Ornon, France.
Guillaume MaucortUniversité de Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, US4, UAR 3420, Bordeaux, France.
Mónica Fernández-MonrealUniversité de Bordeaux, CNRS, INSERM, Bordeaux Imaging Center, US4, UAR 3420, Bordeaux, France.
Florentin DumasLaboratoire de Biogenèse Membranaire, Université de Bordeaux, UMR5200 CNRS, Villenave d'Ornon, France.
Emmanuelle M BayerLaboratoire de Biogenèse Membranaire, Université de Bordeaux, UMR5200 CNRS, Villenave d'Ornon, France.
Yohann BouttéLaboratoire de Biogenèse Membranaire, Université de Bordeaux, UMR5200 CNRS, Villenave d'Ornon, France.
Magali S GrisonLaboratoire de Biogenèse Membranaire, Université de Bordeaux, UMR5200 CNRS, Villenave d'Ornon, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Conventional light microscopy is limited in resolution by the diffraction limit of light, restricting the visualization of the nanoscale organization of biomolecules. Expansion microscopy (ExM) has emerged as a powerful technique to overcome this barrier by physically expanding the specimen embedded in a swellable hydrogel without requiring specialized or high-cost imaging hardware. ExM is widely used in animal models, whereas its application to plant tissues has been challenging due to their multicellularity, in which each cell is encompassed by the rigid cell wall, which resists the expansion forces and prevents isotropic swelling. Here, we describe a robust and optimized ExM protocol specifically designed for

Indexed as

Arabidopsis thaliana rootDeep-tissue imagingExpansion microscopyFluorescence microscopyImmunostainingPlant biologySuper-resolution microscopyTissue clearing

Identifiers

PMID42305134
PMCPMC13266615

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.