Evidence map›Paper›PMID 42304638›Full record

ArticlePest management science2026

Identification of microRNAs associated with defense against Bursaphelenchus xylophilus in Pinus koraiensis and their potential as early diagnostic targets for pine wilt disease.

Xiang Wang, Lu Yu, Yanzhi Yuan, Ke Wei, Yonglin Wang, Xiangcheng Chen, Shan Han, Laifa Wang, Yu Liang, Xi-Zhuo Wang

Abstract read
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In one paragraph

Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Xiang WangKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.ORCID https://orcid.org/0000-0003-0809-2408
Lu YuKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Yanzhi YuanKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Ke WeiKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Yonglin WangBeijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing, China.ORCID https://orcid.org/0000-0003-0993-8102
Xiangcheng ChenCenter for Biological Disaster Prevention and Control, National Forestry and Grassland Administration, Shenyang, China.
Shan HanCollege of Forestry, Sichuan Agricultural University, Chengdu, China.
Laifa WangKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.
Yu LiangCAS Key Laboratory of Forest Ecology and Silviculture, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China.
Xi-Zhuo WangKey Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing, China.

Funding

Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2024MA017)
6 · The paper itself

Abstract

backgroundPine Wilt Disease (PWD), a devastating pine disease caused by Bursaphelenchus xylophilus, severely threatens forest ecosystems. microRNAs (miRNAs) are crucial in host-pathogen interactions, but pine miRNA research is limited and their function in PWD remains unclear. Additionally, PWD lacks effective early diagnostic methods, hindering timely spread control. This study focuses on Pinus koraiensis to explore its miRNAs' role in PWD defense and potential as early diagnostic targets.

resultsWe identified 1026 miRNAs via small RNA high-throughput sequencing, 149 of which were differentially expressed between infected and control groups. Functional enrichment analysis showed that these miRNAs regulate genes related to defense pathways, lipid metabolism, and organic acid metabolism. To overcome PWD diagnostic limitations, we developed the CRISPR/Cas12a-assisted ligation-initiated loop-mediated isothermal amplification (CAL-LAMP) method, targeting novel-miR574 and novel-miR544 with high sensitivity (detection limits as low as 1 amol L

conclusionsThis study developed a novel and rapid diagnostic tool specifically for early-stage PWD-a critical advancement given the long-standing lack of effective early detection methods for the disease. By targeting specific miRNAs, the CAL-LAMP method was capable of detecting target miRNAs in asymptomatic P. koraiensis as early as 10 days post-inoculation under experimental conditions, with high sensitivity and specificity. This early diagnostic capability may provide a useful tool for the timely initiation of disease management measures, although further validation in field settings is necessary to confirm its practical utility. © 2026 Society of Chemical Industry.

Indexed as

MicroRNAsPinusPlant DiseasesRNA, PlantTylenchidaAnimalsMicroRNAsRNA, PlantCRISPRmiRNApine treepine wilt diseasepine wood nematode

Identifiers

PMID42304638
PMCPMC13569323

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.