Evidence map›Paper›PMID 42304343›Full record

ArticleBMC veterinary research2026

Exploratory analysis of gut microbiota and its association with peripheral blood immune cell populations in canine myxomatous mitral valve disease.

Martina Cimerman, Bojan Papić, Alenka Nemec Svete, Jana Avberšek, Darja Kušar, Sara Petek, Miha Bajc, Katka Pohar, Alojz Ihan, Aleksandra Domanjko Petrič

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Article in BMC veterinary research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Martina Cimerman *Veterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, Slovenia.
Bojan Papić *Veterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, Ljubljana, Slovenia.
Alenka Nemec SveteVeterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, Slovenia.
Jana AvberšekVeterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, Ljubljana, Slovenia.
Darja KušarVeterinary Faculty, Institute of Microbiology and Parasitology, University of Ljubljana, Ljubljana, Slovenia.
Sara PetekFaculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia.
Miha BajcFaculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia.
Katka PoharFaculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia.
Alojz IhanFaculty of Medicine, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia.
Aleksandra Domanjko PetričVeterinary Faculty, Small Animal Clinic, University of Ljubljana, Ljubljana, Slovenia. aleksandra.domanjko@vf.uni-lj.si.

Funding

The Slovenian Research and Innovation Agency P4-0053The Slovenian Research and Innovation Agency P4-0092The Slovenian Research and Innovation Agency Young Researcher Core Funding
6 · The paper itself

Abstract

backgroundThere is increasing evidence that gut microbiota is associated with cardiovascular health. In this exploratory study, the 16 S rRNA amplicon sequencing was employed to investigate the composition and differential abundances of bacterial gut microbiota of dogs at different stages of myxomatous mitral valve disease (MMVD): dogs with congestive heart failure (CHF, n = 38), dogs in the preclinical stage of the disease (non-CHF, n = 23) and healthy controls with no apparent heart disease (n = 17). Flow cytometry was performed to quantify T lymphocytes and their subtypes, as well as monocytes, natural killer (NK) cells and B lymphocytes. Concentrations of selected chemokines, N-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP) were also measured. Correlation analysis was performed between immunological parameters and bacterial taxa of the gut microbiota.

resultsAlpha diversity did not differ significantly between the study groups; however, several differentially abundant taxa were identified. Escherichia/Shigella was overabundant in the CHF group and showed a positive correlation with activated T lymphocyte levels, whereas Megamonas was overabundant in the control group and was negatively correlated with monocytes and NT-proBNP levels. Lachnospiraceae was overabundant in the non-CHF group.

conclusionsThese findings suggest that dogs with varying severity of heart disease differ in gut microbiota composition. The observed associations between microbiota profiles, immunological parameters and disease status indicate potential microbiome-immune interactions in disease progression.

Indexed as

Dog DiseasesGastrointestinal MicrobiomeAnimalsDogsFemaleFlow CytometryHeart FailureMaleMitral ValveNatriuretic Peptide, BrainPeptide FragmentsRNA, Ribosomal, 16ST-LymphocytesNatriuretic Peptide, BrainPeptide Fragmentspro-brain natriuretic peptide (1-76)RNA, Ribosomal, 16SCongestive heart failure (CHF)DogsGut microbiotaImmune cellsInflammationMyxomatous mitral valve disease (MMVD)

Identifiers

PMID42304343

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