Evidence map›Paper›PMID 42302129›Full record

ArticleACS nano2026

Multivalent Display of Antimicrobial Peptides on Plant Virus Scaffolds Enhances Killing of Drug-Resistant Bacteria.

Ahmed Ghouneimy, Shahid Chaudhary, Muhammad Tehseen, Ahmed Saleh, Maged Serag, Maya Ayach, Ebtihaj Bukhari, Maazallah Masood, Kosuke Sakashita, Pei-Ying Hong and 3 more

Abstract read
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In one paragraph

Article in ACS nano, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ahmed GhouneimyLaboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology, 4700, Thuwal23955-6900, Saudi Arabia.
Shahid ChaudharyLaboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology, 4700, Thuwal23955-6900, Saudi Arabia.
Muhammad TehseenLaboratory of DNA Replication and Recombination, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Saudi Arabia.
Ahmed SalehLaboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology, 4700, Thuwal23955-6900, Saudi Arabia.
Maged SeragMolecular Imaging and Microscopy Lab, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Saudi Arabia.
Maya AyachImaging & Characterization Core Laboratory, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Kingdom of Saudi Arabia.
Ebtihaj BukhariImaging & Characterization Core Laboratory, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Kingdom of Saudi Arabia.
Maazallah MasoodLaboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology, 4700, Thuwal23955-6900, Saudi Arabia.ORCID 0009-0005-2927-4640
Kosuke SakashitaAnalytical Chemistry Core Lab, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Kingdom of Saudi Arabia.
Pei-Ying HongWater Desalination and Reuse Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Saudi Arabia.ORCID 0000-0002-4474-6600
Satoshi HabuchiMolecular Imaging and Microscopy Lab, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Saudi Arabia.ORCID 0000-0002-6663-2807
Samir M HamdanLaboratory of DNA Replication and Recombination, Division of Biomedical Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal23955-6900, Saudi Arabia.ORCID 0000-0001-5192-1852
Magdy MahfouzLaboratory for Genome Engineering and Synthetic Biology, Division of Biomedical Sciences, King Abdullah University of Science and Technology, 4700, Thuwal23955-6900, Saudi Arabia.ORCID 0000-0002-0616-6365

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multidrug-resistant (MDR) bacteria pose a significant challenge to global health. Antimicrobial peptides (AMPs) have emerged as promising candidates against MDR bacteria due to their rapid and broad-spectrum activity; however, their clinical translation is hindered by compromised activity, toxicity, and poor stability under in vivo conditions. Here, we report the development of RPG (rod-based peptide grids), a plant virus-based antimicrobial platform that harnesses the structural scaffold of high-aspect-ratio Potato virus X (PVX) for the multivalent and modular display of AMPs. Our data show that RPG enhances the efficacy of AMPs by more than 9700-fold, maintaining activity under in vivo salt conditions. RPG eradicates MDR pathogens within 10-30 min, surpassing the efficacy of last-resort antibiotics (vancomycin, tigecycline, and cefiderocol), while exhibiting low measurable cytotoxicity to mammalian cells at high therapeutic doses. Due to structural complexity, RPG demonstrates stability in serum and resistance to proteases. Multivalent display of peptide variants enabled enhanced broad-spectrum killing at low doses. This work establishes plant virus-AMP conjugates as a safe, potent, broad-spectrum antimicrobial platform, offering a versatile strategy for addressing antibiotic resistance.

Indexed as

Anti-Bacterial AgentsAntimicrobial PeptidesPotexvirusMicrobial Sensitivity TestsAnti-Bacterial AgentsAntimicrobial Peptidesantimicrobial peptidesbioproductionMRSAmultidrug resistanceplant virusPVX

Identifiers

PMID42302129
PMCPMC13644466

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.