ArticleSTAR protocols2026
Protocol to isolate and co-culture primary mouse hepatocytes, hepatic stellate cells, and Kupffer cells under physiological conditions.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
3 authors.
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Abstract
With the rising incidence of metabolic diseases, the ability to isolate and co-culture primary hepatic cells has become increasingly critical for investigating liver pathophysiology. We present a protocol to isolate and co-culture primary mouse hepatocytes, hepatic stellate cells, and Kupffer cells. We describe steps for perfusing the liver without a pump, digesting it with collagenase, and separating cells by density centrifugation. We then detail procedures for culturing hepatocytes in a defined, serum-free, plasma-like medium to mimic in vivo conditions.
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Registered trials
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