Protocol update to: Protocol for rapid manipulation of mitochondrial morphology in living cells using inducible counter mitochondrial morphology.

Mayuko Kano, Naoko Onodera, Tomoto Ura, Satone Tanigaki, Teppei Nishino, Takafumi Miyamoto

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Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

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5 · Who and what money

Authors and funding

6 authors.

Mayuko KanoDepartment of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Naoko OnoderaDepartment of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Tomoto UraInstitute of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.

Satone TanigakiDepartment of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Teppei NishinoDepartment of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan.

Takafumi MiyamotoDepartment of Internal Medicine (Endocrinology and Metabolism), Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki 305-8575, Japan; Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan; Center for Cyber Medicine Research, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan. Electronic address: takmi565@md.tsukuba.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Disruption of mitochondrial morphology occurs during various diseases, but the biological significance is not entirely clear. Here, we describe a detailed step-by-step protocol for a chemically inducible dimerization (CID) system-based synthetic protein device, termed inducible counter mitochondrial morphology (iCMM). This system allows artificial manipulation of mitochondrial morphology on a timescale of minutes in living mammalian cells. We also describe an AI-assisted image processing approach. For complete details on the use and execution of this protocol, please refer to Miyamoto et al.

Indexed as

Cell BiologyMicroscopySystems biology

Identifiers

PMID42301815

DOI10.1016/j.xpro.2026.104635

PMCPMC13284483

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.