ArticleChemical communications (Cambridge, England)2026
Azide-functionalized SpCas9 enables generation of site-selective and bioactive Cas9-siRNA conjugates.
Danny Wilbie, Matt Timmers, Asimina Kogkalidou, Erik R Hebels, Igor R Sweet, Roel Maas-Bakker, Esmeralda Bosman, Olivier G de Jong, Tina Vermonden, Enrico Mastrobattista
Abstract read
In one paragraphArticle in Chemical communications (Cambridge, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
10 authors.
Danny WilbieDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.ORCID http://orcid.org/0000-0003-1004-304X Matt TimmersDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.ORCID http://orcid.org/0000-0002-2176-7505 Asimina KogkalidouDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.
Erik R HebelsDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.
Igor R SweetDivision of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht 3508 TB, The Netherlands.
Roel Maas-BakkerDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.
Esmeralda BosmanDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.
Olivier G de JongDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.
Tina VermondenDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.ORCID http://orcid.org/0000-0002-6047-5900 Enrico MastrobattistaDivision of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, Utrecht 3508 TB, The Netherlands. e.mastrobattista@uu.nl.ORCID http://orcid.org/0000-0002-6745-2015 Funding
No grant is acknowledged in the PubMed record.
6 · The paper itselfAbstract
The gene editing enzyme SpCas9 was engineered to display azide on its surface, enabling azide-alkyne click conjugation. As a proof of concept, siRNA functionalized with a reduction sensitive linker and ring-strained alkyne tetramethylthiocycloheptyne sulfoximine was conjugated to SpCas9 on four different residues with varying efficiency and retained protein activity. Conjugation to residue 539 was successful and site-selective while retaining SpCas9 and siRNA bioactivity.
Indexed as
AzidesCRISPR-Associated Protein 9RNA, Small InterferingHumansAzidesCRISPR-Associated Protein 9RNA, Small Interfering
Identifiers
PMID42300182
PMCPMC13232376
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