ArticleLab on a chip2026
Large field of view fluorescence imaging of microfluidic devices with a tandem-lens macroscope.
Article in Lab on a chip, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Microfluidic devices enable high-throughput sample processing with remarkable parallelization and miniaturization. While fluorescence microscopy provides a convenient method for reading out signal from microfluidic assays, commercially-available microscopes impose a fundamental tradeoff between temporal resolution, spatial resolution, and numerical aperture (NA). Spatially tiled imaging enables high-resolution and high-NA imaging over a large area but reduces temporal resolution. Conversely, low magnification, low NA imaging captures large areas in one shot, but typically sacrifices spatial resolution and fluorescence sensitivity. To address this, we introduce an automated transfluorescence tandem-macro-lens optomechanical system (macroscope) capable of sensitive, multi-channel fluorescence imaging over a very large field of view (34 mm diameter, 740 mm
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