Evidence map›Paper›PMID 42299103›Full record

ArticlePest management science2026

Targeting the 20E-responsive miR-2788-Treh1 axis disrupts chitin biosynthesis and ovarian development in the grassland pest Galeruca daurica.

Mingze Shan, Wenbing Zhang, Haichao Wang, Baoping Pang, Haibin Han, Ling Li, Zhijia Huo, Yanmin Shan, Yanhua Ji, Ruiwen Dong and 3 more

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Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

13 authors.

Mingze Shan *Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Wenbing Zhang *Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Haichao WangResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Baoping PangResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.ORCID https://orcid.org/0000-0003-4035-2150
Haibin HanResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Ling LiResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Zhijia HuoResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.
Yanmin ShanInner Mongolia Forestry and Grassland Pest Control and Quarantine Station, Hohhot, China.
Yanhua JiInner Mongolia Forestry and Grassland Pest Control and Quarantine Station, Hohhot, China.
Ruiwen DongSiziwang Grassland Station in Wulanchabu City, Wulanchabu, China.
Zhipeng SunGrassland Station of Xianghuang Banner, Xianghuang Banner, Xilinhot, China.
Yanyan LiResearch Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot, China.ORCID https://orcid.org/0009-0005-1672-4366
Shujing GaoKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot, China.

Funding

Interdisciplinary Research Fund of Inner Mongolia Agricultural University (BR231511)National Key R & D Program of China (2022YFD1401102)Scientific Research Start-up Fund for the Introduction of High-level and Outstanding Doctoral Talents at Inner Mongolia Agricultural University(NDYB2023-37)
6 · The paper itself

Abstract

background20-hydroxyecdysone (20E) coordinates insect reproductive development through extensive metabolic reprogramming. However, the post-transcriptional mechanisms linking hormonal signaling to carbohydrate metabolism during reproductive maturation remain poorly understood in insect pests.

resultsWe identified a novel 20E-miR-2788-Treh1 regulatory axis in Galeruca daurica. Developmental profiling revealed an inverse relationship between miR-2788 and Treh1 expression. Overexpression of miR-2788 or RNA interference (RNAi)-mediated silencing of Treh1 caused trehalose accumulation, reduced expression of chitin biosynthesis genes, and impaired ovarian development. In females, 20E treatment suppressed miR-2788 expression and increased Treh1 expression, thereby promoting ovarian maturation. Similar reproductive defects were induced by the trehalase inhibitor validamycin.

conclusionThese findings suggest that 20E- reproductive miR-2788-Treh1 module contributes, at least in part, to the regulation of reproductive maturation through the -mediate control of trehalose metabolism. The 20E-miR-2788-Treh1 axis represents an important metabolic checkpoint linking endocrine signaling with reproductive development and provides potential molecular targets for environmentally friendly pest management strategies. © 2026 Society of Chemical Industry.

Indexed as

ChitinEcdysteroneInsect ProteinsMicroRNAsMothsAnimalsFemaleOvaryRNA InterferenceChitinEcdysteroneInsect ProteinsMicroRNAs20‐hydroxyecdysoneadult developmentchitin synthesisGaleruca dauricamicroRNApest managementtrehalase

Identifiers

PMID42299103
PMCPMC13569122

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