ArticleBMC plant biology2026
Functional characterization of TONSOKU in Cyathula officinalis reveals its role in DNA damage and root development.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundCyathula officinalis Kuan. (C. officinalis), a perennial medicinal herb endemic to Sichuan, holds significant pharmacological value in traditional medicine, yet its key functional genes governing physiological traits remain unexplored.
resultsCoTSK encodes a nuclear protein containing conserved tetratricopeptide repeat (TPR) and leucine-rich repeat (LRR) domains that phylogenetically clusters with Amaranthaceae homologs. Subcellular localization confirmed its nuclear distribution, consistent with a role in genome maintenance. Functional assays demonstrated that CoTSK expression conferred a 2.5-fold survival advantage under 0.02% methyl methanesulfonate (MMS) stress in E. coli, while showing no tolerance to hydroxyurea (HU), indicating specific protection against alkylation-induced DNA damage. Tissue-specific expression profiling revealed distinct stem-predominant expression in C. officinalis, diverging from the leaf-biased pattern observed in C. capitata (CcTSK). Overexpression of CoTSK partially rescued root developmental defects in Arabidopsis AtTSK mutants, restoring root length to 30-42% of wild-type levels, though stem flattening and rosette proliferation phenotypes remained unrescued.
conclusionsThese findings establish that nuclear-localized CoTSK maintains conserved functions in root development and DNA damage repair while exhibiting distinct stem-predominant expression patterns divergent from its paralog CcTSK. This functional elucidation identifies CoTSK as a molecular target for genetic enhancement of medicinal biomass, particularly through modulation of stem-root developmental coordination, and provides evolutionary insights into TSK family differentiation in Amaranthaceae species.
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