Evidence map›Paper›PMID 42298146›Full record

ArticleApplied microbiology and biotechnology2026

Fusion with immunoglobulin Fc fragment enhances the immunogenicity of the African swine fever virus p30/p54 fusion protein.

Haimi Dang, Yi Ru, Guoqiang Wu, Rongzeng Hao, Jie Xu, Yajun Li, Dongmei Zhao, Chenghui Jiang, Shengnan Han, Xinyu Hu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Haimi DangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yi RuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Guoqiang WuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Rongzeng HaoState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Jie XuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Yajun LiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Dongmei ZhaoState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Chenghui JiangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Shengnan HanState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Xinyu HuState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China.
Xiuping WuSchool of Life Sciences and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
Yang YangState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. yangyang01@caas.cn.
Haixue ZhengState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, China. zhenghaixue@caas.cn.

Funding

Central Public-interest Scientific Institution Basal Research Fund CAAS-ZDRW202409Earmarked Fund for National Pig Industry Technology System CARS-35Fundamental Research Funds for the Central Universities lzujbky-2022-ct02Natural Science Foundation Key Project of Gansu Province 23YFNA0011Science and Technology Department of Gansu Province 23ZDKA0002Youth Science and Technology Talent Innovation Program of Lanzhou 2023-QN-3
6 · The paper itself

Abstract

African swine fever (ASF) is a significant threat to the global pig breeding industry, making the development of a vaccine extremely urgent. In this study, the key antigenic proteins p30 and p54 of African swine fever virus (ASFV) were fused with the Fc fragment of immunoglobulin (IgG) to construct recombinant fusion proteins with strong immunity. Here, we successfully expressed four recombinant fusion proteins comprising the ASFV proteins p30 and p54 (p30-54 and p54-30) alone and the porcine IgG Fc-fused p30 and p54 proteins (p30-54-Fc and p54-30-Fc) using an insect baculovirus expression system. All expressed proteins demonstrated excellent reactivity with specific antibodies, confirming their immunological activity. In vitro studies revealed that the Fc-fused proteins exhibited significantly increased binding affinity to Fcγ receptors (FcγRs) on antigen-presenting cells (APCs). Immunization studies in mice revealed that p30-54-Fc and p54-30-Fc elicited greater humoral and cellular immune responses than p30-54 and p54-30, respectively. Notably, sera from mice immunized with the Fc-fused proteins significantly inhibited ASFV-GFP infection in porcine alveolar macrophages. These results suggest that these ASFV p30-54-Fc and p54-30-Fc recombinant fusion proteins represent promising candidate antigens for the development of an effective ASF subunit vaccine. KEY POINTS: • Fc-fused ASFV p30/p54 proteins elicit stronger humoral and cellular immune responses in mice. • Sera from Fc-fused antigen-immunized mice show enhanced inhibition of ASFV infection in PAMs. • Fc fusion strategy improves immunogenicity of ASFV antigens, supporting promising subunit vaccine candidates.

Indexed as

African Swine FeverAfrican Swine Fever VirusAntigens, ViralImmunoglobulin Fc FragmentsRecombinant Fusion ProteinsViral VaccinesAnimalsAntibodies, ViralAntigen-Presenting CellsBaculoviridaeFemaleImmunity, CellularMiceMice, Inbred BALB CProtein Subunit VaccinesReceptors, IgGAntibodies, ViralAntigens, ViralImmunoglobulin Fc FragmentsProtein Subunit VaccinesReceptors, IgGRecombinant Fusion ProteinsViral VaccinesAfrican swine fever virusFragment of immunoglobulinIgG FcImmunogenicityP30 and p54 proteinsSubunit vaccine

Identifiers

PMID42298146
PMCPMC13500445

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.