Evidence map›Paper›PMID 42297879›Full record

ArticleScientific reports2026

Molecular interactions, solubility, and cytotoxic activity of 4‑hydroxycoumarin in choline chloride‑based acidic deep eutectic solvents.

Mohammad Khorsandi, Hemayat Shekaari, Masoumeh Mokhtarpour, Parisa Akbarzadeh Gondoghdi, Hamed Hamishehkar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mohammad KhorsandiDepartment of Physical Chemistry, University of Tabriz, Tabriz, Iran.
Hemayat ShekaariDepartment of Physical Chemistry, University of Tabriz, Tabriz, Iran. hemayatt@yahoo.com.
Masoumeh MokhtarpourDepartment of Physical Chemistry, University of Tabriz, Tabriz, Iran.
Parisa Akbarzadeh GondoghdiDepartment of Physical Chemistry, University of Tabriz, Tabriz, Iran.
Hamed HamishehkarDrug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study systematically investigated the solubility enhancement of 4-hydroxycoumarin (4HC), a poorly water-soluble pharmaceutical compound, in three novel acidic deep eutectic solvents (ADESs) prepared from choline chloride (ChCl) and acidic hydrogen bond donors encompassing propionic acid (Pro), acetic acid (Ace) and lactic acid (Lac). The solubility measurements were conducted Appling the shake-flask method at 298.15-313.15 K. The ChCl/Pro system exhibited unprecedented more than 500-fold solubility improvement over the pure water, with solubility Increasing in the order: ChCl/Pro > ChCl/Ace > ChCl/Lac. Hansen Solubility Parameters (HSPs) analysis confirmed hydrogen bonding and acidity as dominant dissolution factors. In addition, advanced thermodynamic models (Wilson, e-NRTL, UNIQUAC) accurately correlated experimental solubility data, with the Wilson model achieving ARD percentage values as low as 0.10%. Thermodynamic analysis revealed the dissolution process is endothermic and enthalpy-driven. MTT cytotoxicity assays on HT29 colon cancer cells demonstrated IC₅₀ values in the 20-100 µg/mL range, with differential cytotoxicity correlated to solvent composition. These findings identify ChCl/Pro as the optimal ADES candidate (500-fold solubility enhancement, IC

Indexed as

4-HydroxycoumarinsCholineDeep Eutectic SolventsAntineoplastic AgentsHT29 CellsHumansHydrogen BondingSolubilitySolventsThermodynamics4-HydroxycoumarinsAntineoplastic AgentsCholineDeep Eutectic SolventsSolventsChCl/ProDrug solubilityGreen pharmaceutical solventsHansen solubility parametersMTT assayThermodynamic modeling

Identifiers

PMID42297879
PMCPMC13530267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.