Evidence map›Paper›PMID 42296344›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2026

Regulatory logic underlying neural crest contributions to the head versus the heart.

Shashank Gandhi, Ayyappa Raja Desingu Rajan, Hugo Urrutia, Jillian S Wilson, Irving T C Ling, Ruth Williams, Tatjana Sauka-Spengler, Marianne E Bronner

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shashank GandhiDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.ORCID 0000-0002-4081-4338
Ayyappa Raja Desingu RajanDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.ORCID 0000-0002-0173-0465
Hugo UrrutiaDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.
Jillian S WilsonDepartment of Molecular and Cell Biology, University of California, Berkeley, CA 94720.
Irving T C LingRadcliffe Department of Medicine, University of Oxford, Medical Research Council Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, United Kingdom.
Ruth WilliamsRadcliffe Department of Medicine, University of Oxford, Medical Research Council Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, United Kingdom.
Tatjana Sauka-SpenglerRadcliffe Department of Medicine, University of Oxford, Medical Research Council Weatherall Institute of Molecular Medicine, Oxford OX3 9DS, United Kingdom.ORCID 0000-0001-9289-0263
Marianne E BronnerDivision of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125.ORCID 0000-0003-4274-1862

Funding

Coupling gene regulatory and lineage analysis of the cardiac neural crestR01HL140587 · NHLBI · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI BRONNER, MARIANNE · 2018 to 2021
$2.5M
Clonal analysis of the cranial neural crestR01DE027568 · NIDCR · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI BRONNER, MARIANNE · 2018 to 2022
$2.2M
Investigating the Regulatory Logic Underlying Meningeal Development and TumorigenesisK99DE034827 · NIDCR · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI Ayyappa Raja Desingu Rajan · 2025 to 2026
$325k
Role of BMP signaling in cardiac neural crest developmentK99HL175104 · NHLBI · UNIVERSITY OF CALIFORNIA BERKELEY · PI Shashank Gandhi · 2025 to 2026
$299k
HHS | NIH | NHLBI | Division of Intramural Research (DIR) R01HL14058NHLBI NIH HHS K99 HL175104NHLBI NIH HHS R01 HL140587NIDCR NIH HHS K99 DE034827NIDCR NIH HHS R01 DE027568
6 · The paper itself

Abstract

Neural crest cells exhibit axial-level-specific transcriptional programs, yet the regulatory mechanisms underlying their fate decisions remain unclear. Here, we investigate whether differential transcription factor usage governs cardiac neural crest cell identity. Comparing ATAC-seq profiles of cardiac and cranial neural crest revealed open chromatin regions that were both shared and region-specific. Single-cell RNA sequencing further identified putative target genes, including signaling molecules and receptors. We uncovered two adjacent

Indexed as

HeadHeartNeural CrestAnimalsEnhancer Elements, GeneticGene Expression Regulation, DevelopmentalReceptors, CXCR4Transcription Factor AP-2ZebrafishZebrafish ProteinsCXCR4a protein, zebrafishReceptors, CXCR4Transcription Factor AP-2Zebrafish Proteinscardiaccraniofacialgene regulatory networkheartneural crest

Identifiers

PMID42296344
PMCPMC13271162

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.