ArticleThe Indian journal of medical research2026
Field evaluation of Kyasanur forest disease real-time PCR point-of-care assay.
Article in The Indian journal of medical research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and objectives Kyasanur Forest Disease (KFD) is a tick-borne viral haemorrhagic fever endemic to Western Ghats in India, necessitates rapid and accurate field-based diagnostics for effective outbreak response and timely patient management. Traditional diagnostic methods require laboratory infrastructure, sometimes limiting timely case detection in resource limited settings. We evaluated the diagnostic performance, usability, and operational feasibility of a point of care (POC) assay for detection of KFD virus under field conditions. Methods We assessed a real-time PCR-based portable point-of-care (PoC) test for KFD virus on retrospective clinical samples (n=247) that were obtained from the Goa and Karnataka States in the year 2024. The assay's sensitivity, specificity, and turnaround time (TAT) were assessed in comparison with gold standard real time RT-PCR assay. Operational feasibility and validation was carried out in field laboratory setting in Shivamogga, Karnataka, and North Goa. Results Out of 247 samples analysed, the PoC assay was 97-100% sensitive and 95-98% specific in two sites with less turnaround time than gold standard test. High diagnostic accuracy was demonstrated by the assay which require minimal training for its field application. Interpretation and conclusions The real-time PCR-based PoC assay performed well under field conditions for a reliable and early detection of KFD virus infection. This PoC assay represents a practical and time-efficient diagnostic approach for field use and has the potential to be incorporated into existing KFD surveillance.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.