ArticleMicrobiology spectrum2026
Comparative analysis of wastewater sample processing methods for antimicrobial resistance surveillance.
Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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6 authors.
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Abstract
Antimicrobial resistance monitoring in wastewater represents a key challenge, especially in light of the multiple methods available. Our study assessed the impact of selected sample processing techniques on microbial diversity and antimicrobial resistance gene recovery from wastewater. We analyzed 38 subsamples derived from 12 wastewater samples collected across three wastewater treatment plants over 4 months. We compared the effects of different centrifugation volumes (50, 250 mL), speeds (3,400 × IMPORTANCE: To monitor the prevalence of antimicrobial resistance genes (ARGs) is crucial for anticipating and mitigating the spread of antimicrobial resistance (AMR). Urban wastewater provides community-level data to inform timely interventions. By establishing a method that balances efficiency and feasibility, this protocol represents a step toward a standardized wastewater surveillance (WS) framework for AMR, enhancing our ability to safeguard community health.
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