Integrated microwave-assisted acetic acid pretreatment and endoglucanase-xylanase hydrolysis for nanocrystalline cellulose preparation.

K Y Lim, K Y Foo

Abstract read

In one paragraph

Article in MethodsX, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

2 authors.

K Y LimRiver Engineering and Urban Drainage Research Centre (REDAC), Universiti Sains Malaysia, Tuanku Syed Sirajuddin Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, Penang, Malaysia.

K Y FooRiver Engineering and Urban Drainage Research Centre (REDAC), Universiti Sains Malaysia, Tuanku Syed Sirajuddin Engineering Campus, Seri Ampangan, 14300 Nibong Tebal, Penang, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study presents a high-efficiency extraction strategy for nanocrystalline cellulose (NCC) from tangerine peel as a model lignocellulosic biomass, using sequential microwave (MW)-assisted acetic acid (AA) pretreatment and dual-enzyme (endoglucanase and xylanase) hydrolysis. MW-assisted AA pretreatment (50 % AA, 100 °C, 10 min) achieves rapid volumetric heating and enhanced solvent penetration, and selective removal of hemicellulose, lignin and pectin components, while preserving crystalline cellulose domains, generating a highly accessible substrate for enzymatic processing. Subsequent hydrolysis with endoglucanase and xylanase (25 U/g substrate (TP-CFs) for endoglucanase and 50 U/g substrate (TP-CFs) for xylanase, 50 °C, 5 h, pH 4.8) depolymerizes amorphous cellulose regions and residual hemicellulosic barriers under mild conditions, resulting in NCC with uniform nano-fibrillar morphology (length 603.48 nm, diameter 9.23 nm, aspect ratio 65.36), high crystallinity (64.35 %), enhanced thermal stability (T

Indexed as

Acetic acidEndoglucanaseEnzymatic hydrolysisMicrowaveNanocrystalline celluloseOrganosolv pretreatmentTangerine peelXylanase

Identifiers

PMID42292863

DOI10.1016/j.mex.2026.103961

PMCPMC13253094

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