ArticleBiochemistry and biophysics reports2026
Automated method for determining the zeta potential of red blood cells via motion tracking in microelectrophoresis.
Article in Biochemistry and biophysics reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Membrane-Targeted Consequences of Acetaminophen Toxicity and Off-Target Effects of Antimicrobial Peptides on Host Cell Membranes.International journal of molecular sciences · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The surface charge of red blood cells (RBCs), commonly expressed as zeta potential, is a key biophysical parameter influencing RBC aggregation, deformability, and microcirculatory behavior. Despite its physiological and clinical relevance, routine assessment of RBC zeta potential is limited by methodological complexity and poor reproducibility of conventional microelectrophoretic techniques. In this study, we present an automated method for determining the zeta potential of individual RBCs based on computer-assisted tracking of cell motion during microelectrophoresis. Video recordings of RBC movement in an electric field were analyzed using automated spot detection and trajectory reconstruction, allowing electrophoretic mobility and zeta potential to be calculated at the single-cell level. Application of the method to RBCs from healthy donors revealed reproducible zeta potential distributions, while additionally revealing distinct subpopulations of cells with markedly reduced surface charge. The effect of electric field direction on zeta potential was systematically evaluated, demonstrating the contribution of diffuse layer relaxation effects and supporting the use of bidirectional measurements to improve accuracy. Furthermore, incubation of RBCs with gramicidin S, trypsin, and neuraminidase induced pronounced and statistically significant reductions in zeta potential, confirming the sensitivity of the method to alterations in membrane composition and sialic acid content. Overall, the proposed automated microelectrophoretic approach enables objective, high-resolution analysis of RBC electrokinetic properties and overcomes key limitations of manual techniques. This method holds promise for studying red blood cell aging, membrane damage, and drug-cell interactions, and may contribute to the development of novel biophysical biomarkers relevant to hematological and systemic disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.