ArticleBioscience nanotechnology2026
L-DNA calibrators for PCR amplicon characterization.
Article in Bioscience nanotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
While real-time polymerase chain reaction (PCR) is powerful on its own, its utility is markedly improved by post-PCR techniques like melt analysis and target quantification. We have previously utilized L-DNA stereoisomers matching the sequences of the reaction primers and target amplicon to increase PCR sensitivity and specificity in the presence of interferents. In this report we describe using L-DNA sequences as internal calibrators to confirm amplicon specificity and estimate initial target concentration. An Supplementary Information: The online version contains supplementary material available at 10.1186/s44331-026-00022-w.
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