Evidence map›Paper›PMID 42290285›Full record

ArticleDevelopment (Cambridge, England)2026

Basal cytoplasmic calcium levels regulate C. elegans germ stem cell proliferation.

Fariha J Khan, Parva M Vyas, Aahana N Shankaran, Alexandra C Wells, Corrissa J Velder, Rabia N Kaya, Anuj R Nagula, Edward T Kipreos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors.

Fariha J KhanDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Parva M VyasDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Aahana N ShankaranDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Alexandra C WellsDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Corrissa J VelderDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Rabia N KayaDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Anuj R NagulaDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.
Edward T KipreosDepartment of Cellular Biology, University of Georgia, Athens, GA 30602, USA.ORCID 0000-0001-9650-3558

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Folate-directed signaling in C. elegansR01GM134359 · NIGMS · UNIVERSITY OF GEORGIA · PI KIPREOS, EDWARD T. · 2020 to 2023
$1.2M
NIGMS NIH HHS R01 GM134359NIGMS NIH HHS R01GM134359NIH HHS P40 OD010440University of Georgia
6 · The paper itself

Abstract

The Caenorhabditis elegans germline is one of the foremost models for the study of germ cell biology. We created an integrated ratiometric calcium reporter to assess calcium levels in the C. elegans germ line. The reporter reveals changes in basal cytoplasmic calcium levels during hermaphrodite sexual development. Oogenic cells have elevated calcium levels, while spermatogenic cells have significantly reduced levels of calcium. There are a small number of germ cells in the bend region of mid-to-late L4 hermaphrodite germ lines with elevated calcium levels that are correlated with the spermatogenesis-to-oogenesis transition. We identified GON-2 as a calcium channel that is required for the maintenance of normal calcium levels in the germline. Significantly, basal calcium levels correlate with germ cell proliferation. Starvation and inactivation of pro-proliferation signaling pathways reduce basal calcium levels, while proliferating germline tumor cells have elevated basal calcium levels. Experimentally altering calcium levels leads to a concomitant increase or decrease in the proliferation rate of germ stem cells. These results imply that basal cytoplasmic calcium acts as a rheostat to regulate germ stem cell proliferation.

Indexed as

Caenorhabditis elegansCalciumCytoplasmGerm CellsStem CellsAnimalsCaenorhabditis elegans ProteinsCalcium ChannelsCalcium SignalingCell ProliferationMaleSignal TransductionSpermatogenesisCaenorhabditis elegans ProteinsCalciumCalcium ChannelsBasal calciumCell proliferationGerm cellsGermline developmentSex determination

Identifiers

PMID42290285
PMCPMC13380967

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.