Evidence map›Paper›PMID 42289824›Full record

ArticleMicrobiologyOpen2026

Acetate-Linked Energy Metabolism as a Determinant of Early Haemophilus influenzae Infection Fitness.

Marufa Nasreen, Jennifer Hosmer, Saurab Kishore Munshi, Riya Joshi, Ayaho Yamamoto, Horst J Schirra, Peter Sly, Alastair G McEwan, Ulrike Kappler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Marufa NasreenSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.
Jennifer HosmerSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.
Saurab Kishore MunshiSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.
Riya JoshiSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.ORCID https://orcid.org/0000-0002-3433-8136
Ayaho YamamotoChild Health Research Centre, The University of Queensland, South Brisbane, Queensland, Australia.ORCID https://orcid.org/0000-0001-5143-4608
Horst J SchirraSchool of Environment and Science, Griffith Institute for Drug Discovery, Griffith University, Nathan, Queensland, Australia.
Peter SlyChild Health Research Centre, The University of Queensland, South Brisbane, Queensland, Australia.ORCID https://orcid.org/0000-0001-6305-2201
Alastair G McEwanSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.
Ulrike KapplerSchool of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, Queensland, Australia.ORCID https://orcid.org/0000-0002-2642-1319

Funding

National Health and Medical Research Council (NHMRC) 1158451National Health and Medical Research Council (NHMRC) 2019058
6 · The paper itself

Abstract

Acetate is a major metabolic end-product of Haemophilus influenzae and is produced via the phosphotransacetylase-acetate kinase (Pta-AckA) pathway, which also generates adenosine triphosphate (ATP). Despite its importance, the contribution of this pathway to H. influenzae physiology and virulence remains poorly understood. Here, we have investigated the individual roles of AckA and Pta for these processes by generating nonpolar single-gene knockouts in a chronic obstructive pulmonary disease (COPD)-isolate strain (Hi2019) and characterized their metabolic and infection phenotypes. Both mutants exhibited significant growth impairments under microaerobic and anaerobic conditions, with reductions in growth rate of up to 50% compared with the wild type. Loss of AckA or Pta decreased ATP levels to ~ 50% of wild-type values and caused marked overoxidation of the NAD

Indexed as

AcetatesEnergy MetabolismHaemophilus InfectionsHaemophilus influenzaeAcetate KinaseAdenosine TriphosphateAnimalsBiofilmsEpithelial CellsGene Knockout TechniquesHumansMiceOxidative StressPhosphate AcetyltransferasePulmonary Disease, Chronic ObstructiveVirulenceAcetate KinaseAcetatesAdenosine TriphosphatePhosphate AcetyltransferaseacetateHaemophilus influenzaeinfectious diseasesmetabolismredox biology

Identifiers

PMID42289824
PMCPMC13265617

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.