Identification and characterization of a novel leaderless bacteriocin toyoncin T35 from Bacillus toyonensis.

Xiaofeng Zhang, Haihua Wang, Chenxu He, Xiaojing Zhang, Xuesi Li, Chunmei Pan

Abstract read

In one paragraph

Article in Microbial cell factories, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Xiaofeng Zhang *Henan University of Animal Husbandry and Economy, Zhengzhou, China. zhangxiaof78@126.com.

Haihua Wang *Henan University of Animal Husbandry and Economy, Zhengzhou, China.

Chenxu HeHenan Xinghan Biotechnology Co., Ltd., Puyang, China.

Xiaojing ZhangHenan University of Animal Husbandry and Economy, Zhengzhou, China.

Xuesi LiHenan University of Animal Husbandry and Economy, Zhengzhou, China.

Chunmei PanHenan University of Animal Husbandry and Economy, Zhengzhou, China.

Funding

the Doctoral Initiation Fund from Henan University of Animal Husbandry and Economy 2021HNUAHEDF006the Doctoral Initiation Fund from Henan University of Animal Husbandry and Economy 20250116005the Key Disciplines of Henan Province (2023-2027) Number 311, Code 0951the Science and Technique Foundation of Henan Province 242102111018the Science and Technique Foundation of Henan Province;the Doctoral Initiation Fund from Henan University of Animal Husbandry and Economy 232102320297

6 · The paper itself

Abstract

backgroundAntibiotics residues and antibiotics resistance have become a serious threat to human health. There is an urgent need for the development of antibiotic alternatives. Bacteriocins are considered one of the alternative to antibiotics because of their different antimicrobial mechanisms from antibiotics. Therefore, mining novel bacteriocins is of great significance for the development of antibiotic alternatives.

resultsA novel aureocin A53-like leaderless bacteriocin, toyoncin T35, was identified in B. toyonensis T35. Toyoncin T35 was composed of 48 amino acid residues and its molecular weight was 5491.9950 Da. Toyoncin T35 exhibited strong antimicrobial activity against foodborne pathogens B. cereus (MIC, 0.71 µM), L. monocytogenes (MIC, 0.35 µM) as well as pathogenic bacteria S. suis (MIC, 2.84 µM). In addition, toyoncin T35 could inhibit B. cereus spore outgrowth, and possessed broad acid-base adaptability (pH 2-10) along with high heat tolerance (below 100 °C). Toyoncin T35 has no hemolysis activity and cytotoxicity even at a high concentration of 91.00 µM.

conclusionsThe novel leaderless bacteriocin toyoncin T35 has the potential to become an antibiotic alternative for treating diseases caused by foodborne pathogens B. cereus, L. monocytogenes, and pathogenic bacteria S. suis.

Indexed as

Anti-Bacterial AgentsBacillusBacteriocinsAmino Acid SequenceHemolysisMicrobial Sensitivity TestsMolecular WeightAnti-Bacterial AgentsBacteriocinsBacillus toyonensisLeaderless bacteriocinToyoncin T35

Identifiers

PMID42289680

DOI10.1186/s12934-026-03044-4

PMCPMC13491596

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.