ArticleScientific reports2026
Nanozymatic platform for dual colorimetric and fluorimetric sensing of H₂O₂ and glucose using Ni-CoWO₄ nanoparticles.
Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Hydrogen peroxide (H₂O₂) plays a crucial role in both biological and industrial systems. Excessive H₂O₂ can lead to oxidative stress, cellular damage, and process-related issues in manufacturing. For this reason, fast and sensitive detection methods are needed. In this study, Ni-CoWO₄ nanoparticles, belonging to the bimetallic nanozymes family, were used as catalysts to promote the oxidation of ortho-phenylenediamine (OPD) by H₂O₂. Polyoxometalates are renowned for their structural diversity, large surface area, and robust redox activity, making them exceptional candidates for catalytic applications. Ni-CoWO₄ was prepared using a hydrothermal method and characterized using SEM-EDX, XRD, and FT-IR techniques. The nanoparticles exhibited an even distribution of nickel and cobalt within a multi-metallic framework, resulting in numerous active sites and a high surface-to-volume ratio. Compared to the system without the catalyst, Ni-CoWO₄ significantly improved the reaction rate, produced a stronger yellow color, and reduced the oxidation time. The reaction was monitored by visible spectrophotometry, spectrofluorimetry, and smartphone-based colorimetry. A linear range of 0.5-150 µM and a limit of detection of 485 nM for H
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