Evidence map›Paper›PMID 42288989›Full record

ArticleRapid communications in mass spectrometry : RCM2026

Toward Metabolomics Analyses With Combined Capillary Vibrating Sharp-Edge Spray Ionization and Atmospheric Pressure Chemical Ionization.

Madison Pursell, Adefolake Ojanuga, Chandrima Banerjee, Ephraim Ezeigbo, Mohammad Rahman, Vikum K Dewasurendra, Thomas B Hughart, Matthew B Johnson, Mst Nigar Sultana, Xiaoting Guo and 5 more

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Article in Rapid communications in mass spectrometry : RCM, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Madison PursellDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Adefolake OjanugaDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Chandrima BanerjeeDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Ephraim EzeigboDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Mohammad RahmanDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Vikum K DewasurendraDepartment of Physics, West Virginia University, Morgantown, West Virginia, USA.
Thomas B HughartDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Matthew B JohnsonDepartment of Physics, West Virginia University, Morgantown, West Virginia, USA.
Mst Nigar SultanaDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Xiaoting GuoInvibragen, Inc., Morgantown, West Virginia, USA.
Gabriel AlexanderDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Priya PatelDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.
Peng LiDepartment of Chemistry, Lehigh University, Bethlehem, Pennsylvania, USA.
Justin LegleiterDepartment of Biochemistry, Molecular Biology, & Biotechnology, University of Nevada Reno, Reno, Nevada, USA.
Stephen J ValentineDepartment of Chemistry, West Virginia University, Morgantown, West Virginia, USA.ORCID https://orcid.org/0000-0003-4360-2928

Funding

National Science Foundation 2305137
6 · The paper itself

Abstract

rationaleThe varied properties of metabolomics components dictate the tools used in mass spectrometry (MS) characterization including the selection of the ionization source. However, the ionization of select compounds limits characterization efficiency. A simple setup employing vibrating sharp-edge spray ionization (VSSI) accesses two distinct ionization processes that can be performed cyclically during LC.

methodsThe MS approach described here couples analyte sample nebulization by VSSI with corona discharge to achieve ionization via an atmospheric pressure chemical ionization (APCI)-like process. A 28-component small molecule standard mixture as well as a C. elegans metabolite extract are analyzed to determine potential for utilization in metabolomics experiments.

resultsFor the standard panel, the cVSSI-APCI approach is shown to better ionize half of all compounds compared to ESI. For the metabolomics extract, cVSSI-APCI is shown to significantly outperform ESI for multiple compounds across a range of reversed-phase LC solution conditions, while ESI is shown to outperform cVSSI-APCI for other species. Manual cycling between field-enabled cVSSI and cVSSI-APCI ionization processes results in relatively rapid equilibration to distinct ionization processes with high reproducibility in compound ionization.

conclusionCycling between ionization processes is accomplished on a timescale suitable with longer timescale LC runs. To obtain sufficient cycling rates for high-throughput metabolomics experiments (~10 to 20 min) will require the use of high-voltage, high-power pulser electronics. The preliminary results suggest such an approach will be successful for online analyses.

Indexed as

MetabolomicsSpectrometry, Mass, Electrospray IonizationAnimalsAtmospheric PressureCaenorhabditis elegansdual technique ionization sourcemass spectrometrymetabolomics

Identifiers

PMID42288989
PMCPMC13264966

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.