Evidence map›Paper›PMID 42288657›Full record

ArticleScientific reports2026

System for study of diverse cellular composition in alveolar rhabdomyosarcoma.

Guak-Kim Tan, Kavya Kannan, Hollis Wright, Zu-Yuan Qian, Sonja D Chen, Gillian M St John, Sofia M Tosoni, Brian P Rubin, Melvin Lathara, Charles Keller

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Guak-Kim TanChildren's Cancer Therapy Development Institute, 9025 NE Von Neumann Drive Ste 110, Hillsboro, OR, 97006, USA. guakkim@gmail.com.
Kavya KannanDatma, Beaverton, OR, 97005, USA.
Hollis WrightChildren's Cancer Therapy Development Institute, 9025 NE Von Neumann Drive Ste 110, Hillsboro, OR, 97006, USA.
Zu-Yuan QianChildren's Cancer Therapy Development Institute, 9025 NE Von Neumann Drive Ste 110, Hillsboro, OR, 97006, USA.
Sonja D ChenThe Ohio State University College of Medicine and Nationwide Children's Hospital, Columbus, OH, 43205, USA.
Gillian M St JohnChildren's Cancer Therapy Development Institute, 9025 NE Von Neumann Drive Ste 110, Hillsboro, OR, 97006, USA.
Sofia M TosoniDatma, Beaverton, OR, 97005, USA.
Brian P RubinRobert J. Tomsich Department of Pathology and Laboratory Medicine, Cleveland Clinic, Cleveland, OH, 44195, USA.
Melvin LatharaDatma, Beaverton, OR, 97005, USA.
Charles KellerChildren's Cancer Therapy Development Institute, 9025 NE Von Neumann Drive Ste 110, Hillsboro, OR, 97006, USA. charles@cc-tdi.org.

Funding

NCI NIH HHS R01CA258120NIH HHS R01CA258120
6 · The paper itself

Abstract

Alveolar rhabdomyosarcoma (ARMS) is an aggressive pediatric soft tissue sarcoma often driven by PAX3::FOXO1 or PAX7::FOXO1 fusions, for which therapy can be limited if metastatic. ARMS pathology shows pronounced cellular heterogeneity, including malignant subpopulations at distinct myogenic differentiation stages. Detailed subpopulation transcriptional programs and microenvironmental influences are of ongoing interest biologically and therapeutically. Using a genetically engineered mouse model and single-cell RNA sequencing (scRNA-seq), we characterized tumor composition and cellular states. Histology and immunohistochemistry confirmed diverse morphologies and differential expression of myogenic markers. scRNA-seq identified 17 clusters, including 11 malignant, immune and stromal subpopulations. Transcriptomic analysis revealed precursor-like cells expressed epithelial/EMT-like programs, while rhabdomyoblast-like cells upregulated extracellular matrix and mesenchymal regulators. Allografts in immunodeficient mice showed that immune context shapes cellular composition. Lineage-specific reporters and Symphony mapping validated spatial segregation of precursor and rhabdomyoblast-like cells. These findings demonstrate transcriptionally distinct malignant subpopulations within an immune-influenced microenvironment. These ARMS genetically engineered mouse model and allograft systems provides a platform to study tumor heterogeneity and drug responses.

Indexed as

Rhabdomyosarcoma, AlveolarAnimalsDisease Models, AnimalGene Expression ProfilingGene Expression Regulation, NeoplasticHumansMiceSingle-Cell AnalysisSingle-Cell Gene Expression AnalysisTumor MicroenvironmentAlveolar rhabdomyosarcomaGenetically engineered mouse modelGenetic profiling

Identifiers

PMID42288657
PMCPMC13522354

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.