Abiotic synthesis of RNase-resistant phosphodiester and pyrophosphate-linked polymers via thermodynamically controlled wet-dry cycling.

Povilas Simonis, Hannah V Rheinstädter, Viktoras Masevičius, Ralph E Pudritz, Katherine E Bujold, Maikel C Rheinstädter

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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Povilas SimonisDepartment of Functional Materials and Electronics, Vilnius University, Vilnius, Lithuania. povilas.simonis@ftmc.lt.

Hannah V RheinstädterDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada.

Viktoras MasevičiusDepartment of Functional Materials and Electronics, Vilnius University, Vilnius, Lithuania.

Ralph E PudritzDepartment of Physics and Astronomy, McMaster University, Hamilton, ON, Canada.

Katherine E BujoldDepartment of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada. bujoldk@mcmaster.ca.

Maikel C RheinstädterDepartment of Physics and Astronomy, McMaster University, Hamilton, ON, Canada. rheinstadter@mcmaster.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the abiotic synthesis of structurally modified, RNase-resistant oligonucleotides with phosphodiester backbones from non-activated mononucleotides of adenosine monophosphate (AMP) and uridine monophosphate (UMP), and pyrophosphate linked D-ribose-5'-phosphate polymers under simulated wet-dry cycling conditions. These polymers were generated in a dedicated simulation chamber, under well controlled and reproducible thermodynamic conditions. The polymers lead to diffuse bands in electrophoresis gels using fluorescence detection with SYBR Gold at an excitation wavelength of 365 nm, a key spectral signature that allows us to distinguish them from standard nucleic acids. We show that these polymers are stable against enzymatic degradation by RNase 1, DNase 1, and DBR1, however partially degrade in alkaline hydrolysis. Evidence for polymerization was obtained in UV-vis and IR spectra. SAXS suggests aggregates in solution of approximately 120 nucleotide and ribose phosphate units.

Indexed as

DiphosphatesOligonucleotidesPolymersRibonucleasesAdenosine MonophosphateThermodynamicsUridine MonophosphateAdenosine MonophosphateDiphosphatesOligonucleotidesPolymersRibonucleasesUridine MonophosphateAbiotic synthesisEnvironmental simulatorNucleic acidsOligonucleotidesPrebiotic chemistryRibose polymersWet-dry cycles

Identifiers

PMID42288570

DOI10.1038/s41598-026-56548-9

PMCPMC13518880

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