Evidence map›Paper›PMID 42287536›Full record

ArticleApplied biochemistry and biotechnology2026

Collagenase from Lysinibacillus sphaericus: Production, Optimization and Application in Adipose-Derived Stem Cell Isolation.

Zhen-Zhen Wang, Dai Xue, Jian-Ying Qian, Kang Wang, Ling-Feng Xu, Xu-Dong Ma, Nan Xie, Chang Su, Jin-Song Gong, Zheng-Hong Xu and 1 more

Abstract read
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In one paragraph

Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Zhen-Zhen WangSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Dai XueWuxi Children's Hospital, Wuxi, 214000, PR China. 337855521@qq.com.
Jian-Ying QianSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China. jackieqian@163.com.ORCID http://orcid.org/0009-0006-7032-351X
Kang WangSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Ling-Feng XuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Xu-Dong MaCytori Therapeutics LLC, Shanghai, 201802, PR China.
Nan XieCytori Therapeutics LLC, Shanghai, 201802, PR China.
Chang SuSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Jin-Song GongSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.
Zheng-Hong XuCollege of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, PR China.
Jin-Song ShiSchool of Life Sciences and Health Engineering, Jiangnan University, Wuxi, 214122, PR China.

Funding

National Key Research and Development Program of China 2023YFA0914500National Natural Science Foundation of China 32301283
6 · The paper itself

Abstract

Collagenase, predominantly found in microorganisms, has significant potential for tissue separation and primary cell extraction. In this study, a collagenase Col51 derived from Lysinibacillus sphaericus JN-51 was isolated from sludge samples collected from aquaculture markets and slaughterhouses. Col51 was expressed in Bacillus subtilis (SCK6) and purified using a Ni-NTA column. Enzymatic characterization revealed that the enzyme exhibits optimal activity at 37 °C and pH 7.5. The presence of 10 mM Mg²⁺ and Ca

Indexed as

Adipose TissueBacillaceaeCell SeparationCollagenasesStem CellsBacillus subtilisHydrogen-Ion ConcentrationCollagenasesAdipose-derived stem cellsBacillus subtilisCollagenaseLysinibacillus sphaericus

Identifiers

PMID42287536

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.