Characterization of the ctgf Promoter from Nibea coibor and its Response to TGF-β1 During Collagen Synthesis in Swim Bladder Cells.

Ailing Zou, Yuying Yuan, Lingling Zhu, Yu Zhang, Lanfei Xiao, Xiaobo Wen, Fan Lin

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Article in Marine biotechnology (New York, N.Y.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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7 authors.

Ailing ZouGuangdong Provincial Key Laboratory of Marine Biology, Institute of Marine Sciences, Shantou University, 243 Daxue Road, Shantou, 515063, China.

Yuying YuanGuangdong Provincial Key Laboratory of Marine Biology, Institute of Marine Sciences, Shantou University, 243 Daxue Road, Shantou, 515063, China.

Lingling ZhuGuangdong Provincial Key Laboratory of Marine Biology, Institute of Marine Sciences, Shantou University, 243 Daxue Road, Shantou, 515063, China.

Yu ZhangGuangdong Provincial Key Laboratory of Marine Biology, Institute of Marine Sciences, Shantou University, 243 Daxue Road, Shantou, 515063, China.

Lanfei XiaoCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.

Xiaobo WenCollege of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China.

Fan LinGuangdong Provincial Key Laboratory of Marine Biology, Institute of Marine Sciences, Shantou University, 243 Daxue Road, Shantou, 515063, China. linfan@stu.edu.cn.

Funding

China Postdoctoral Science Foundation 2025M773132Guangdong province key project 2023TQ07A509National Natural Science Foundation of China 32373108National Science Foundation of China U25A20724Natural Science Foundation of Guangdong Province 2024A1515010564Research on breeding technology of candidate species for Guangdong modern marine ranching 2024-MRB-00-001Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province STKJ202209036

6 · The paper itself

Abstract

Connective tissue growth factor (ctgf) is a multifunctional protein that plays a crucial role in promoting collagen (the most abundant protein in organism) synthesis in vertebrates. However, its transcriptional regulation mechanism is poorly understood, especially in teleosts. In this study, we characterized the ctgf promoter (3,185 bp) from a marine teleost, the chu's croaker (Nibea coibor), and elucidated its response to TGF-β1 during collagen synthesis in swim bladder cells. Bioinformatics analysis revealed conserved cis-elements (TATA box, SP-1, Smad-3, NF-κB) within the promoter. Truncation assays identified a core functional region (-785 ~ + 166 bp). Notably, site-directed mutagenesis showed that mutation of SP-1 binding site enhanced the promoter activity, while mutations in binding sites of Smad-3 (-290 bp) and NF-κB (-262 bp) suppressed its activity. To demonstrate its correlation with collagen synthesis, swim bladder cells stably transfected with ctgf promoter drived EGFP vector (pctgf-EGFP) was constructed. It was further found that the promoter activity was up-regulated in concert with collagen synthesis promoted by TGF-β1 in swim bladder cells. These findings could advance our understanding of the transcriptional regulation of ctgf, and establish a technical platform for further screening natural compounds promoting collagen synthesis in fish.

Indexed as

Air SacsCollagenConnective Tissue Growth FactorFish ProteinsPerciformesPromoter Regions, GeneticTransforming Growth Factor beta1AnimalsBase SequenceBinding SitesGene Expression RegulationMutagenesis, Site-DirectedNF-kappa BCollagenConnective Tissue Growth FactorFish ProteinsNF-kappa BTransforming Growth Factor beta1Cis-regulatory elementCollagen synthesisctgf promoterNibea coiborSwim bladderTGF-β1

Identifiers

PMID42287469

DOI10.1007/s10126-026-10657-8

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.