Identification of adducts formed between phosphatidylcholine and mustard agents.

Matti A Kjellberg, Noora-Kaisa Rantanen, Shishir Jaikishan, Susanne K Wiedmer, Hanna Hakulinen

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Matti A KjellbergFinnish Institute for Verification of the Chemical Weapons Convention (VERIFIN), Department of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, POB 55, 00014, Helsinki, Finland. matti.kjellberg@helsinki.fi.

Noora-Kaisa RantanenFinnish Institute for Verification of the Chemical Weapons Convention (VERIFIN), Department of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, POB 55, 00014, Helsinki, Finland.

Shishir JaikishanDepartment of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, POB 55, 00014, Helsinki, Finland.

Susanne K WiedmerDepartment of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, POB 55, 00014, Helsinki, Finland.

Hanna HakulinenFinnish Institute for Verification of the Chemical Weapons Convention (VERIFIN), Department of Chemistry, University of Helsinki, A.I. Virtasen Aukio 1, POB 55, 00014, Helsinki, Finland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sulphur and nitrogen mustards are two groups of highly toxic blistering agents. Upon human exposure, these agents produce characteristic biomarkers, including alkylated proteins and DNA. In contrast, lipids, which comprise a varied class of biomolecules, have received little attention as potential alkylation targets of mustard agents. In this work, we demonstrate that sulphur and nitrogen mustards bind covalently to 1-palmitoyl-2-oleoyl-glycero-sn-3-phosphocholine in model membrane systems. We use ultra-high-performance liquid chromatography-high-resolution tandem mass spectrometry to characterise the structures of these novel analytes. Additionally, alkylated phosphatidylcholines were detectable in human plasma spiked with trace concentrations of sulphur mustard. Plasma samples were prepared by liquid-liquid extraction and analysed by targeted multi-reaction monitoring ultra-high-performance liquid chromatography-tandem mass spectrometry. Our data suggest that these novel alkylated lipids may function as biomarkers of mustard agent exposure.

Indexed as

Chemical Warfare AgentsMustard CompoundsMustard GasPhosphatidylcholinesAlkylationBiomarkersChromatography, High Pressure LiquidHumansLiquid Chromatography-Mass SpectrometryTandem Mass Spectrometry1-palmitoyl-2-oleoylphosphatidylcholineBiomarkersChemical Warfare AgentsMustard CompoundsMustard GasPhosphatidylcholinesBiomarkerChemical warfare agentLiquid chromatography-mass spectrometryMustard gasPhosphatidylcholinePhospholipid

Identifiers

PMID42287400

DOI10.1007/s00216-026-06600-4

PMCPMC13388424

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.