Evidence map›Paper›PMID 42286561›Full record

ArticleBMC biology2026

Engineered targeted enhancement of translation in mammalian cell.

Junzhe Liu, Yonghui Lv, Zhihong Zhou, Linzhen Huang, Kunjian Lei, Zewei Tu, Chuandong Gong, Zhansheng Fang, Chong Wang, Juan Wang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Junzhe Liu *Department of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Yonghui Lv *Department of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Zhihong Zhou *Department of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Linzhen HuangDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Kunjian LeiDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Zewei TuDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Chuandong GongDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Zhansheng FangDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Chong WangDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China.
Juan WangEast China Institute of Digital Medical Engineering, Shangrao, China.
Xingen ZhuDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China. ndefy89006@ncu.edu.cn.
Minhua YeDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China. yeminhua2007@126.com.
Shigang LvDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China. lvsg1982@163.com.
Kai HuangDepartment of Neurosurgery, The 2nd Affiliated Hospital,, Jiangxi Medical College, Nanchang University, Nanchang, China. kaihuang@ncu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWhile the central dogma outlines DNA-to-protein information flow, existing gene regulators mainly target transcription. Here, we developed the λN-Guided RNA Targeting System (λGRTS), a CRISPR-independent platform enhancing mammalian mRNA translation via specific translation-guiding RNAs (tgRNAs).

resultsλGRTS integrates λN (high-affinity BoxB binder), mutated eIF4E1 (ablated non-specific 5' cap binding, retains TIC recruitment), and auxiliary factors (HuR for dsRNA stabilization, PABP and RRM2-RRM3 for mRNA closed loops). Optimized 22-nt tgRNAs (targeting 58 bp upstream of mRNA ATG in 5' UTR) and NES-tagged proteins maximized efficacy. λGRTS outperformed dCasRX (smaller ~ 50 kDa vs. ~ 150 kDa, single tgRNA vs. crRNA-tracrRNA), boosting functional proteins (e.g., GFP). It activated P53/PTEN, suppressing GBM cell proliferation, inducing G1 arrest, and reducing invasion in vitro. In vivo, lentiviral λGRTS inhibited orthotopic GBM in nude mice, extended survival by approximately 35-40 days, with IHC confirming P53/PTEN upregulation. Mass spectrometry showed no off-target effects. Cross-species tests (human, mouse, bovine cells) validated broad applicability via conserved eIF4E1.

conclusionsλGRTS offers a specific, safe translation-centric tool for gene regulation and oncology research.

Indexed as

Protein BiosynthesisAnimalsCell Line, TumorHumansMiceMice, NudeTgRNATranslation enhancementλGRTSλN-BoxB

Identifiers

PMID42286561
PMCPMC13540907

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.