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ArticleCurrent microbiology2026

First Report of Colletotrichum siamense (Ascomycota, Glomerellaceae) Causing Severe Leaf Spots and Blights Disease on Annona reticulata in Kerala, India, Investigated Through Phenetic and Molecular Phylogenetic Analysis.

Shambhu Kumar, Thottungathara Subran Kripa, Kalathil Thodi Mufeeda, Shivannegowda Mahadevakumar, Raghvendra Singh

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Article in Current microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Shambhu KumarForest Pathology Department, KSCSTE-Kerala Forest Research Institute, Peechi, Thrissur, 680653, Kerala, India. skumartaxon@gmail.com.ORCID http://orcid.org/0000-0001-9534-9309
Thottungathara Subran KripaForest Pathology Department, KSCSTE-Kerala Forest Research Institute, Peechi, Thrissur, 680653, Kerala, India.ORCID http://orcid.org/0000-0002-6328-1327
Kalathil Thodi MufeedaForest Pathology Department, KSCSTE-Kerala Forest Research Institute, Peechi, Thrissur, 680653, Kerala, India.ORCID http://orcid.org/0009-0001-2625-2746
Shivannegowda MahadevakumarAndaman & Nicobar Regional Centre, Botanical Survey of India, Haddo, Port Blair, 744102, India.ORCID http://orcid.org/0000-0002-8611-7503
Raghvendra SinghCentre for Advanced Study in Botany, Institute of Science, Banaras Hindu University, Varanasi, 221005, Uttar Pradesh, India.ORCID http://orcid.org/0000-0002-8672-6149

Funding

SERB, DST, Government of India CRG/2019/005014
6 · The paper itself

Abstract

Custard apple (Annona reticulata) is a fast-growing semi-evergreen tree well known for its nutritional and medicinal properties to treat against various kinds of diseases. During investigation, the leaf spots and blight disease was observed on A. reticulata in Kerala, India. The causative agent was isolated and identified as Colletotrichum siamense, based on symptomatic inspection, cultural morphology, microscopic characteristics, and concatenate multigene molecular phylogenetic analysis using internal transcribed spacer region (ITS), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-tubulin (TUB2) genes. Koch's postulates were fulfilled through in vitro pathogenicity and confirmed the pathogenic nature of C. siamense. To the best on our knowledge, and consulting with upto-date literature, this is the first report of C. siamense causing leaf spots and blights disease on A. reticulata from India. The study highlights the need for surveillance and effective management of this foliar disease in A. reticulata within tropical ecosystems to protect its cultivation and commercial value.

Indexed as

AnnonaColletotrichumPhylogenyPlant DiseasesDNA, FungalDNA, Ribosomal SpacerIndiaPlant LeavesSequence Analysis, DNATubulinDNA, FungalDNA, Ribosomal SpacerTubulin

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.