Evidence map›Paper›PMID 42285963›Full record

ArticleNature communications2026

Energy-translation coupling limits anaerobic yeast growth.

Yongbo Wang, Yu Huang, Yazhi Liu, Lifan Chen, Xiaofei Yu, Zilong Shao, Yu Chen, Feiran Li, Sizhe Qiu, Yong Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Yongbo Wang *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yu Huang *State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yazhi LiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Lifan ChenState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Xiaofei YuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Zilong ShaoState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Yu ChenState Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.ORCID http://orcid.org/0000-0003-3326-9068
Feiran LiInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.ORCID http://orcid.org/0000-0001-9155-5260
Sizhe QiuState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID http://orcid.org/0000-0002-1936-1223
Yong WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.ORCID http://orcid.org/0000-0001-5541-3978
Yingping ZhuangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Guan WangState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. guanwang@ecust.edu.cn.ORCID http://orcid.org/0000-0003-2341-3054

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding how nutrient-specific limitations shape anaerobic metabolism in Saccharomyces cerevisiae is essential for defining the physiological limits of yeast growth. Integrating chemostat physiology, multi-omic profiling, and targeted metabolic engineering under strictly anaerobic conditions, we show that yeast maintains a conserved maximum glucose uptake (~14 mmol/gDW/h) under carbon (C), nitrogen (N), and phosphorus (P) limitation, while distinct regulatory bottlenecks constrain maximal growth rate: ATP insufficiency under C and P limitation, and aminoacyl-tRNA synthetase scarcity under N limitation. Under these stresses, S. cerevisiae reallocates proteomic resources toward anabolic functions, with nutrient-specific phosphorylation networks compensating for translational stress, most pronounced under N limitation. Building on these insights, a "push-pull" strategy enhancing energy supply (VMA3) and translational capacity (WRS1) increased the maximal anaerobic growth rate by 27.2%, 47.5% and 52.5% under C, N, and P limitation, respectively. These findings reveal energy-translation coupling as the central determinant of anaerobic growth limits and provide a framework for rational strain engineering.

Indexed as

Energy MetabolismProtein BiosynthesisSaccharomyces cerevisiaeAdenosine TriphosphateAnaerobiosisCarbonGlucoseMetabolic EngineeringNitrogenPhosphorusProteomicsSaccharomyces cerevisiae ProteinsAdenosine TriphosphateCarbonGlucoseNitrogenPhosphorusSaccharomyces cerevisiae Proteins

Identifiers

PMID42285963
PMCPMC13458083

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.