ArticleThe Plant journal : for cell and molecular biology2026
The fluorescence-activating and absorption-shifting tag (FAST), a versatile protein marker for live plant cell imaging.
Article in The Plant journal : for cell and molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
The development of real-time imaging has progressed spectacularly in recent years, largely due to the availability of multiple fluorophores with distinct spectral properties. This progress enables the simultaneous imaging of an increasing number of targets, providing access to complex biological processes occurring during development, signaling, and metabolism. In this study, we successfully tested the transfer of three different fluorescence-activating and absorption-shifting tags (FAST), originally developed in animal systems and yeast, to plants. These small (14 kDa) proteins associate non-covalently and reversibly with specific chemicals known as fluorogens, generating bright fluorescence signals that can be reversibly eliminated by washing. This property offers versatile applications. Green- and Red-FAST proteins were successfully used to label proteins targeted to several plant cellular compartments. In contrast, the latest generation of FAST proteins, identified in Rheinheimera sp. A13L (RspA), which accepts multiple fluorogens and offers broad versatility in excitation and emission spectra, was used with a split FAST version to demonstrate cytosolic protein-protein interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.