ArticleACS nano2026
Storage Buffer Composition Impacts Internal Structure, Freeze-Thaw Stability, and Transfection Efficiency of mRNA-Lipid Nanoparticles.
Article in ACS nano, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- A Ferrous-Supply-Regenerating Lipid Nanoparticle Integrating RNAi Induces Ferroptosis for Cancer Therapy.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- mRNA lipid nanoparticle vaccines: current status, challenges and future prospects.Molecular biomedicine · 2026Review
Corrections and comments
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Authors and funding
20 authors.
Funding
Abstract
Messenger RNA (mRNA) lipid nanoparticles (mRNA-LNPs) are central to emerging vaccines and therapeutics, but their wide implementation is constrained by limited endosomal escape and instability during long-term storage and freezing. While buffers are routinely optimized to prevent instability, the impact of buffer on the internal structural organization of LNPs and, consequently, their delivery efficiency remain unresolved. Here, we study the impact of storage in Tris, histidine, and citrate buffers for mRNA-LNPs formulated with LP-01, MC3, and SM-102 ionizable lipids. We demonstrate that storage buffer identity and concentration govern mRNA-LNP internal ordering before and after freeze-thaw and are thus critical parameters for engineering high-performance formulations. Deconvoluting ordered phases into an mRNA-lipid region and excess lipid region reveals the importance of excess ionizable lipid behavior in enhancing endosomal escape. Prior to freezing, citrate buffer enhances transfection efficiency by promoting a transition to the fusogenic inverse hexagonal (H
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Registered trials
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