ArticleJournal of clinical microbiology2026
Improving the culture surrounding tracheal aspirates in mechanically ventilated children.
Article in Journal of clinical microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Authors and funding
10 authors.
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Abstract
Interpretation of tracheal aspirate cultures is limited by contamination with normal respiratory flora and inconsistency in collection, processing, and reporting. Our aim was to both decrease waste and improve the quality and interpretability of tracheal aspirate cultures by implementing laboratory-driven Gram stain rejection criteria. We reviewed prior tracheal aspirate specimens and cultures, examined the current literature, and gathered input from stakeholders to inform potential Gram stain rejection criteria. Rates of tracheal aspirates collected, cultured, and rejected pre-intervention (9 November 2019-2 April 2023) and post-intervention (3 April 2023-28 February 2024) were compared. We implemented culture rejection criteria and rejected specimens with Gram stains demonstrating moderate to heavy epithelial cells and/or no organisms/three or more organism morphologies. The median rate of tracheal aspirates cultured decreased from 7.7 to 2.4 per 100 ventilator days (
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