Evidence map›Paper›PMID 42283125›Full record

ArticleNucleic acids research2026

Visualizing cellular G-quadruplexes with a bioinspired chemiluminescent probe.

Pengfei Wang, Xiaoyu Li, Zhichun Wang, Ziyue Guan, Jean-Louis Mergny, Jing Yang, Mingpan Cheng

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Pengfei WangState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.ORCID 0000-0001-5471-5595
Xiaoyu LiState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.
Zhichun WangState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.
Ziyue GuanState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.
Jean-Louis MergnyLaboratoire d'Optique et Biosciences, Ecole Polytechnique, CNRS, INSERM, Institut Polytechnique de Paris, Palaiseau 91120, France.ORCID 0000-0003-3043-8401
Jing YangState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.
Mingpan ChengState Key Laboratory of Natural Medicine, Department of Biomedical Engineering, School of Engineering, China Pharmaceutical University, Nanjing 211198, China.ORCID 0000-0003-1282-0076

Funding

CNRSEcole PolytechniqueInsermJiangsu Province Frontier Technology Research and Development Program BF2024075Jiangsu Specially Appointed Professor Program, Innovation and Entrepreneurship (Shuangchuang) Program of Jiangsu Province JSSCTD202447National Foreign Experts Program S20250128National Natural Science Foundation of China 22307143National Natural Science Foundation of China NSFC82202237Natural Science Foundation of Jiangsu Province, China BK20252073Natural Science Foundation of Jiangsu Province, China BKA20231007
6 · The paper itself

Abstract

Fluorescence imaging of G-quadruplexes (G4s) is fundamentally limited by autofluorescence and phototoxicity from external excitation. Chemiluminescence, which generates light internally via chemical reaction, offers a promising solution. Here, we report the first chemiluminescent probe, PCL, for selective G4 visualization in vitro and in cells. PCL conjugates the high-affinity G4 ligand PhenDC3 with a reactive oxygen species (ROS)-activatable imidazopyrazinium chemilumophore. Crucially, we demonstrate that PCL's excitation-free mechanism enables high-contrast, low-background imaging of G4s in living cells. This work establishes a novel chemiluminescent paradigm for visualizing G4s with minimal perturbation.

Indexed as

Fluorescent DyesG-QuadruplexesLuminescent MeasurementsHeLa CellsHumansReactive Oxygen SpeciesFluorescent DyesReactive Oxygen Species

Identifiers

PMID42283125
PMCPMC13261070

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.