ArticlebioRxiv : the preprint server for biology2026
A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.
Shuyuan Yang, Alex James McDonald, Xiaoyu Lu, Vincent Villette, Noura Hakam, Mario Galdamez, Tanmayee Torne-Srivas, Gregory Foran, Xiaoyu Dong, Matthew Shorey and 19 more
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In one paragraphArticle in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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5 · Who and what moneyAuthors and funding
29 authors.
Shuyuan YangDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, TX, 77005, USA.ORCID 0000-0001-5977-9782 Vincent VilletteInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.ORCID 0000-0002-8210-1678 Noura HakamDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Tanmayee Torne-SrivasDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.ORCID 0000-0003-0859-7748 Xiaoyu DongDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Nima BaviliDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Tom MaslianitsynaWavefront-engineering Microscopy Group, Vision Institute, UMR S968 Inserm, UMR 7210 CNRS, UM 80 Sorbonne University, 75012, Paris, France.
Rémi FournelWavefront-engineering Microscopy Group, Vision Institute, UMR S968 Inserm, UMR 7210 CNRS, UM 80 Sorbonne University, 75012, Paris, France.
Emiliano RonzittiWavefront-engineering Microscopy Group, Vision Institute, UMR S968 Inserm, UMR 7210 CNRS, UM 80 Sorbonne University, 75012, Paris, France.ORCID 0000-0002-3146-7880 Jonathan BradleyInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.ORCID 0000-0002-6933-1153 Daniela Gaspar SantosInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.ORCID 0000-0002-6744-5026 Lindsey RanDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Ming HuDepartment of Neuroscience, Baylor College of Medicine, Houston, TX, 77030, USA.
Valentina EmilianiWavefront-engineering Microscopy Group, Vision Institute, UMR S968 Inserm, UMR 7210 CNRS, UM 80 Sorbonne University, 75012, Paris, France.ORCID 0000-0003-2992-9510 Laurent BourdieuInstitut de Biologie de l'École Normale Supérieure (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, Paris F-75005, France.ORCID 0000-0003-0195-8758 Funding
Tumor BiologyP30CA125123 · NCI · BAYLOR COLLEGE OF MEDICINE · PI Suzanne AW Fuqua · 2007 to 2026
$73.9MTissue Analysis & Molecular Imaging CoreP30DK056338 · NIDDK · BAYLOR COLLEGE OF MEDICINE · PI Hashem B El-Serag · 2001 to 2026
$28.3MMolecular Engineering of Natural Light-Gated Chloride Channels for Optogenetic InhibitionU01NS118288 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI SPUDICH, JOHN LEE, ST-PIERRE, FRANCOIS · 2020 to 2023
$5.2MENABLING SUBMILLISECOND-TIMESCALE TWO-PHOTON RECORDING OF VOLTAGE DYNAMICS IN THREE DIMENSIONS IN VIVOU01NS133971 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2023 to 2026
$4.0MDesigning and deploying an expanded color palette of voltage indicators engineered for multiphoton microscopyU01NS113294 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS, TOLIAS, ANDREAS · 2019 to 2021
$3.7MEngineering photostable fluorescent proteins and biosensors using transcriptomic mining and massive-throughput single-cell screeningR01EB032854 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS · 2022 to 2025
$2.4MThe Spatial and Temporal Scale of Neuromodulation in Mouse Sensory CortexRF1NS128901 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI REIMER, JACOB · 2022 to 2022
$2.3MEngineering designer probes for imaging membrane potential: novel parts, architectures, and platformsR01EB027145 · NIBIB · BAYLOR COLLEGE OF MEDICINE · PI ST-PIERRE, FRANCOIS · 2019 to 2022
$2.2MOptimizing FACED for robust large-scale activity recordingU01NS137449 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI NA Ji · 2024 to 2026
$2.1MAdaptive optical microscopy for high-accuracy recording of neural activity in vivoU01NS118300 · NINDS · UNIVERSITY OF CALIFORNIA BERKELEY · PI JI, NA · 2021 to 2023
$1.7MRed-shifted voltage indicators for two-photon all-optical physiology and multi-spectral imagingR01NS136027 · NINDS · BAYLOR COLLEGE OF MEDICINE · PI Jacob Reimer, Francois St-Pierre · 2024 to 2026
$1.7MACQUISITION OF THE YOKOGAWA CV8000 HIGH THROUGHPUT SPINNING DISK MICROSCOPE AND ROBOTICSS10OD030414 · OD · BAYLOR COLLEGE OF MEDICINE · PI MANCINI, MICHAEL A. · 2022 to 2022
$1.4MNCI NIH HHS P30 CA125123NIBIB NIH HHS R01 EB027145NIBIB NIH HHS R01 EB032854NIDDK NIH HHS P30 DK056338NIH HHS S10 OD030414NINDS NIH HHS R01 NS128901NINDS NIH HHS R01 NS136027NINDS NIH HHS R01 NS146023NINDS NIH HHS R01 NS146078NINDS NIH HHS R34 NS132045NINDS NIH HHS RF1 NS128901NINDS NIH HHS U01 NS113294NINDS NIH HHS U01 NS118288NINDS NIH HHS U01 NS118300NINDS NIH HHS U01 NS133971NINDS NIH HHS U01 NS137449
6 · The paper itselfAbstract
Genetically encoded voltage indicators (GEVIs) enable minimally invasive, cell-type-specific optical measurements of neuronal membrane potential with millisecond temporal resolution. Red-shifted GEVIs are especially advantageous because they permit spectral multiplexing with complementary sensors and enable all-optical circuit interrogation in combination with blue-light-activated opsins. Despite these advantages, existing red GEVIs remain poorly suited for in vivo use due to limited performance under two-photon (2P) excitation, the predominant modality for deep-tissue imaging. Here, we introduce VADER1, a red GEVI that overcomes this limitation and enables reliable spike detection in vivo under 2P illumination. Under 2P excitation, VADER1 supports extended voltage imaging with both random-access and resonant-scanning microscopy, enables recordings from neurons as deep as cortical layer 5, and allows dual-color imaging with calcium indicators. By filling a critical spectral gap, VADER1 enables integrated optical measurements of fast electrical activity alongside other neural signals and establishes a foundation for two-photon all-optical electrophysiology.
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PMID42282847
PMCPMC13251918
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