Evidence map›Paper›PMID 42282740›Full record

ArticlebioRxiv : the preprint server for biology2026

Protocol for assessing lysosomal ion channel function in mammalian cells using lysosomal patch-clamp technique.

Yayu Wang, Lily Yeh Jan

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Yayu WangDepartment of Physiology, University of California at San Francisco; San Francisco, CA 94158, USA.ORCID 0000-0003-3976-3817
Lily Yeh JanDepartment of Physiology, University of California at San Francisco; San Francisco, CA 94158, USA.

Funding

The TMEM16 Family of Ion Channels and Lipid ScramblasesR35NS122110 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LILY JAN · 2021 to 2026
$3.2M
NINDS NIH HHS R35 NS122110
6 · The paper itself

Abstract

This protocol describes manual whole-endolysosome patch-clamp recordings from pharmacologically or genetically enlarged endolysosomes in cultured mammalian cells. Steps include vesicle enlargement, fabrication and fire-polishing of high-resistance pipettes, mechanical dissection and isolation of enlarged vesicles, giga-seal formation, and configuration to whole-endolysosome modes. For complete details on the use and execution of this protocol, please refer to Wang et al

Identifiers

PMID42282740
PMCPMC13252132

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.