Evidence map›Paper›PMID 42282701›Full record

ArticlebioRxiv : the preprint server for biology2026

Megalin (LRP2), prenatal betamethasone, and injury susceptibility in the developing kidney.

C Nakum, B Bull, S Yarlagadda, S Indugula, K Stowers, K VanDenHeuvel, J T Ference-Salo, J Beamish, A Volz, J E Robinson and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

C NakumDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
B BullDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
S YarlagaddaDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
S IndugulaDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
K StowersDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
K VanDenHeuvelDivision of Pathology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
J T Ference-SaloDepartment of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
J BeamishDepartment of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
A VolzDivision of Experimental Hematology & Cancer Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
J E RobinsonDepartment of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
D K SinghDivision of Clinical and Translational Pharmacology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
B PrasadDepartment of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
M P SchuhDivision of Nephrology and Hypertension, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.ORCID 0000-0001-7854-8138

Funding

Bridging the gap of late gestation human nephrogenesis using a non-human primate modelK08DK131259 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI SCHUH, MEREDITH · 2022 to 2024
$574k
The transcriptional, regulatory, and spatially defined changes from mid to late gestation in a non-human primate modelR03DK141897 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI SCHUH, MEREDITH · 2025 to 2025
$241k
NIDDK NIH HHS K08 DK131259NIDDK NIH HHS R03 DK141897
6 · The paper itself

Abstract

Preterm infants undergo postnatal nephrogenesis and are often exposed to gentamicin (gent). Mothers at risk of preterm birth receive betamethasone (beta) to accelerate fetal lung development. Gent cytotoxicity occurs in proximal tubules (PT) after LRP2-mediated endocytosis. The objective of this study was to evaluate the impact of proximal tubular maturation, impacted by both age and prenatal beta, on injury susceptibility and nephron number. Pups were given toxic gent dosing (100mg/kg) or saline intraperitoneal x 5 days during nephrogenesis (P0-4) or tubular maturation (P6-10). This was repeated with maternal exposure to beta to evaluate impact of beta on injury. Proteomic analyses identified non-monotonic increased LRP2 protein abundance at P10, correlating with increased injury to gent exposure from P6-10 relative to P0-4. P10 pups exposed to prenatal beta had significantly more LRP2 relative to controls, which correlated to more injury after gent exposure at P6-P10. Only those exposed to prenatal beta with P6-10 gent demonstrated ~50% nephron reduction. This study supports that tubular maturation is a critical period of vulnerability to gentamicin correlating to LRP2 expression. Prenatal corticosteroids increase the severity of acute and chronic injury in this highest risk exposure group.

Identifiers

PMID42282701
PMCPMC13252413

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.