Evidence map›Paper›PMID 42282655›Full record

ArticlebioRxiv : the preprint server for biology2026

G quadruplex DNA facilitates a pervasive path to homologous recombination.

Bhushan L Thakur, Priyanka Basak, Simran Khurana, Shalu Sharma, Yen-Hsi Lai, Tai Nguyen, Vijayalalitha Ramanarayanan, Xianzhen Zhao, Pedro J Batista, Travis H Stracker and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Bhushan L ThakurDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Priyanka BasakDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Simran KhuranaRadiation Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Shalu SharmaRadiation Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Yen-Hsi LaiDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Tai NguyenLaboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD, USA.
Vijayalalitha RamanarayananDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Xianzhen ZhaoDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Pedro J BatistaLaboratory of Cell Biology, National Cancer Institute, NIH, Bethesda, MD, USA.ORCID 0000-0003-1055-2354
Travis H StrackerRadiation Oncology Branch, National Cancer Institute, NIH, Bethesda, MD, USA.
Philipp OberdoerfferDepartment of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Funding

Vaccine Response and Immunotherapeutics SWGP30AI094189 · NIAID · JOHNS HOPKINS UNIVERSITY · PI Anna Palmer Durbin · 2012 to 2026
$67.0M
Regulation of RNA biogenesis and function by RNA modificationsZIABC011766 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI DE OLIVEIRA RODRIGUES BATISTA, PEDRO · 2017 to 2025
$10.6M
Tousled like kinase signaling in cancerZIABC012010 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI STRACKER, TRAVIS · 2020 to 2025
$5.7M
Epigenetic mechanisms controlling single-stranded DNA lesion sensitivity and mutagenesisR01CA285725 · NCI · JOHNS HOPKINS UNIVERSITY · PI Philipp Oberdoerffer · 2024 to 2026
$2.6M
Probing the mechanisms that control DNA repair pathway choice.R35GM153484 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Philipp Oberdoerffer · 2024 to 2026
$1.5M
MoFlo to MoFlo XDP Upgrade for BSL3 SortingS10OD016315 · OD · JOHNS HOPKINS UNIVERSITY · PI MARGOLICK, JOSEPH B. · 2013 to 2013
$233k
Intramural NIH HHS ZIA BC011766Intramural NIH HHS ZIA BC012010NCI NIH HHS R01 CA285725NIAID NIH HHS P30 AI094189NIGMS NIH HHS R35 GM153484NIH HHS S10 OD016315
6 · The paper itself

Abstract

Homologous recombination (HR) requires efficient homology search and strand invasion, yet how homologous templates are identified within nuclear space remains unclear. Here, we identify G-quadruplex (G4) DNA structures as pervasive effectors of template strand invasion and uncover the G4 helicase DHX36 as a potent suppressor of this process. DHX36 loss stabilizes G4s, enhances HR, and accelerates repair of replication-associated DNA breaks. G4-mediated HR depends on the non-canonical strand invasion factors RAD51AP1, WDR48, and USP1, and requires G4 motifs within the homologous repair template. DHX36 loss partially restores HR and PARP inhibitor resistance in BRCA1-deficient cells, while promoting aberrant recombination and Alternative Lengthening of Telomeres (ALT). Together, our findings establish dynamic G4 regulation as a key determinant of homology search, genome maintenance, and recombination fidelity.

Identifiers

PMID42282655
PMCPMC13251956

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.