Evidence map›Paper›PMID 42282651›Full record

ArticlebioRxiv : the preprint server for biology2026

High-avidity TCR signaling induces a distinct KLR-positive exhaustion state in human tumor-infiltrating CD8 T cells associated with immunotherapy response.

Riley D Z Mullins, Jesse M Zaretsky, Thomas F Barrett, Emily Stoller, Porter Bischoff, Ann Marie Egloff, Cody Huffman, Salma Ramadan, Amna Ramadan, Jessica Ley and 22 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors.

Riley D Z MullinsDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Jesse M ZaretskyThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Thomas F BarrettDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Emily StollerDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Porter BischoffDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Ann Marie EgloffDepartment of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Cody HuffmanDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Salma RamadanDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Amna RamadanDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Jessica LeyThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Katherine SchwetyeThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Daniel MillerThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Jennifer De Los SantosThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Anthony J ApicelliThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Nikhil RammohanThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Wade ThorstadThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Peter OppeltThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Brendan J KnappThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Christine AuberleThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Ben WahleDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Jason T RichDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Ryan S JacksonDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Patrik PipkornDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Paul A ZolkindDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Richard A HarbisonDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.
Takeshi EgawaDepartment of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.
Nathan SinghDivision of Oncology, Section of Cellular Therapies, Washington University School of Medicine, St. Louis, MO, USA.
Robi D MitraDepartment of Genetics, Washington University School of Medicine, St. Louis, MO, USA.
Sana D KaramDepartment of Radiation Oncology, Washington University School of Medicine, St. Louis, MO, USA.
Ravindra UppaluriDepartment of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA.
Douglas R AdkinsThe Robert Ebert and Greg Stubblefield Head and Neck Tumor Center at Siteman Cancer Center, St. Louis, MO, USA.
Sidharth V PuramDepartment of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St. Louis, MO, USA.

Funding

TRAINING IN THE IMMUNOBIOLOGY AND MOLECULAR CELL BIOLOGY OF CANCERT32CA009547 · NCI · WASHINGTON UNIVERSITY · PI ROBERT DAVID SCHREIBER · 1986 to 2026
$12.1M
Washington University Paul Calabresi K12 Career Development Award for Clinical OncologyK12CA167540 · NCI · WASHINGTON UNIVERSITY · PI John F. Dipersio, Ramaswamy Govindan · 2012 to 2026
$11.7M
The transcription factor c-MYC in lymphocyte expansion and restriction of stemnessR01AI130152 · NIAID · WASHINGTON UNIVERSITY · PI Takeshi Egawa · 2017 to 2026
$4.6M
Deciphering epigenetically-regulated pathways to improve targeted therapy for invasion and metastasis in head and neck cancerR01DE032865 · NIDCR · WASHINGTON UNIVERSITY · PI Robi D Mitra, Sidharth Venkata Puram · 2023 to 2026
$2.4M
Molecular mechanisms of CD8 T cell fate decision instructed by cytokine signalingR01AI176664 · NIAID · WASHINGTON UNIVERSITY · PI Takeshi Egawa · 2024 to 2026
$1.8M
Dissecting hybrid epithelial-mesenchymal states in head and neck cancerR01DE032371 · NIDCR · WASHINGTON UNIVERSITY · PI Sidharth Venkata Puram · 2023 to 2026
$1.6M
Understanding programs of invasion and metastasis in head and neck cancerK08CA237732 · NCI · WASHINGTON UNIVERSITY · PI PURAM, SIDHARTH VENKATA · 2019 to 2023
$984k
RNA modification and the regulation of partial EMT in head and neck cancerR21DE031072 · NIDCR · WASHINGTON UNIVERSITY · PI PURAM, SIDHARTH VENKATA · 2021 to 2022
$433k
The Determinants of CD8 T cell Dysfunction in Oral Cavity Squamous Cell CarcinomaF31DE032562 · NIDCR · WASHINGTON UNIVERSITY · PI MULLINS, RILEY DALTON ZALE · 2023 to 2024
$70k
NCI NIH HHS K08 CA237732NCI NIH HHS K12 CA167540NCI NIH HHS T32 CA009547NIAID NIH HHS R01 AI130152NIAID NIH HHS R01 AI176664NIDCR NIH HHS F31 DE032562NIDCR NIH HHS R01 DE032371NIDCR NIH HHS R01 DE032865NIDCR NIH HHS R21 DE031072
6 · The paper itself

Abstract

Tumor-specific exhausted CD8 T cells (Tex) adopt diverse phenotypes across human cancers, but the drivers of this heterogeneity remain poorly understood. Using flow cytometry and single-cell RNA and T cell receptor (TCR) sequencing of 106,667 tumor-infiltrating CD8 T cells from head and neck squamous cell carcinoma (HNSCC) tumors, we identified and validated three Tex subsets, each with distinct clonotypes: (1) Tex-Conv, expressing conventional exhaustion genes; (2) Tex-CCR6, distinguished by

Identifiers

PMID42282651
PMCPMC13252105

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.