ArticleRSC chemical biology2026
Thio-modification effects on mRNA translation using a PureCap-based capping method.
Article in RSC chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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12 authors.
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Abstract
Controlling translational efficiency, stability, and innate immune recognition remains a critical challenge for the practical application of mRNA therapeutics, and chemical modification of nucleic acids is widely employed as an effective strategy to address these issues. Sugar modifications exemplified by 4'-thio substitution and phosphate-backbone modifications exemplified by phosphorothioate (PS) substitution can alter the behavior of nucleic acids. In this study, to introduce 4'-thio substitution and PS substitution at the +1 and +2 nucleotides of the 5' end of mRNA, we synthesized a series of cap analogs based on the PureCap method that we previously established, which enables preparation of highly pure capped mRNA for reliable evaluation of cap modification effects. Using the resulting cap analogs, mRNAs were prepared by
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