Single-contig bacterial genomes recovered from cattle fecal metagenomes at farms with variable antibiotic use.

Cattle feces represent a complex microbial reservoir with implications for animal health and the environmental dissemination of microorganisms and antibiotic resistance genes. Metagenomic studies have shown that cattle fecal communities are dominated by Bacillota and Bacteroidota, whereas low-abundance taxa, including potential pathogens, often remain underrepresented due to methodological detection limits. Here, we present 84 single-contig, medium- to high-quality metagenome-assembled genomes (MAGs) recovered from cattle feces after enrichment for bacteria able to grow in acetate-supplemented minimal medium. The MAGs were classified within the phyla Actinomycetota (20 MAGs), Bacillota (5), Bacteroidota (21), Patescibacteriota (5), and Pseudomonadota (33), with 41 MAGs representing putative novel taxa at species to family level. Nineteen MAGs carried antibiotic resistance genes and six MAGs were assigned to opportunistic pathogenic species. This dataset thus provides a genomic resource for studies of bacterial diversity and antimicrobial resistance at the animal-environment interface within a One Health framework.